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Paper
Title

Prevalence of Vibrio parahaemolyticus species and frequency of tdh pathogenic gene in strains isolated from Persian Gulf fish and shrimp

Year: 1399
COI: BIOCONF21_0801
Language: EnglishView: 29
نسخه کامل این Paper ارائه نشده است و در دسترس نمی باشد

Authors

Mohasseneh Ghari - Department of Biotechnology, Faculty of Biological Sciences and Technology, Shahid Ashrafi Esfahani University, Esfahan, Iran
Afsaneh Karamostaji - Infectious and Tropical Diseases Research Center, Hormozgan Health Institute, Hormozgan University of Medical Sciences, Bandar Abbas, Iran
Maryam Sadat Mirbagheri Firoozabad - Department of Biology, Faculty of Science, Yazd University, Yazd, Iran
Sayed Mahdi Ghasemi - Department of Biotechnology, Faculty of Biological Sciences and Technology, Shahid Ashrafi Esfahani University, Esfahan, Iran

Abstract:

Vibrio are gram-negative, curved bacilli, halotolerant bacteria that live in sea waters. The present study was performed to determine the prevalence of Vibrio parahaemolyticus species and the frequency of tdh pathogenic gene in Vibrio parahaemolyticus isolated from fresh fish and shrimp samples and salt.In this descriptive cross-sectional study, ۱۱۸ samples consisting of fresh and salted fish and shrimp were collected from different regions of the Persian Gulf and immediately transferred to the lab. They were enriched and ۱ g of the samples were poured into tubes containing alkaline peptone water and transferred for ۶ hours at ۳۷ ° C. After this incubation period, the samples were occulated on TCBS and incubated for ۲۴ hours at ۳۷ °C. Initial identification of Vibrio parahaemolyticus based on the observation of green colonies that are not capable of consuming sucrose sugar and were Gram negative curved bacteria. In Vibrio parahaemolyticus, tdh gene was also identified on isolates using PCR. The results of the present study indicate the high prevalence of Vibrio parahaemolyticus in samples of fresh shrimp and salted fish. The results of biochemical tests showed that ۳۶ out of ۱۱۸ samples (۳۰.۵%) were infected with Vibrio parahaemolyticus. Therefore, it is suggested to use PCR method as a safe, accurate and fast test for detecting Vibrio species in seafood, in order to control the health of seafood in terms of the presence of Vibrio species.

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This Paper COI Code is BIOCONF21_0801. Also You can use the following address to link to this article. This link is permanent and is used as an article registration confirmation in the Civilica reference:

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Ghari, Mohasseneh and Karamostaji, Afsaneh and Mirbagheri Firoozabad, Maryam Sadat and Ghasemi, Sayed Mahdi,1399,Prevalence of Vibrio parahaemolyticus species and frequency of tdh pathogenic gene in strains isolated from Persian Gulf fish and shrimp,21th National & 9th International Congress on Biology,https://civilica.com/doc/1260767

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Type of center: موسسه غیرانتفاعی
Paper count: 518
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