Bioinformatics study of Streptomyces genome by antiSMASH web server

Streptomyces produce two-thirds of the polyketide synthase antibiotics. The Streptomyces genome encodes three types of polyketide synthase (type I, type II, and type III). In this study, antiSMASH Web server was used to identify secondary metabolites, and evaluate the gene clusters of non-ribosomal polycystic syntheses and polyketide synthase. The ۱۸ strains of Streptomyces genome sequence were obtained from the NCBI database in Gene Bank formate and analyzed by antiSMASH Web server for surveying the secondary metabolites production. Streptomyces hygroscopicus subsp. hygroscopicus had the most of PKS type I gene clusters and ۵ of the strains had PKS type I gene clusters. Type I PKSs are multifunctional enzymes organized into modules, each of which has a set of distinct and non-repetitive activities responsible for catalyzing a cycle of increasing poly-chain. The cathode is synthesized by ۶-dioxycyarithromycin B (DEBS) for the biosynthesis of reduced polypectides such as erythromycin. The PKSs type I gene cluster with a set of enzymes that are responsible for the biosynthesis of the base structure of polyketide synthase. Among the ۱۸ strains of Streptomyces, Streptomyces globisporus and Streptomyces libani lacked PKS type II and PKS type III gene cluster respectively. Eleven strains had only PKS type III gene cluster. The results indicate that Streptomyces have a very high potential for the production of secondary metabolites and valuable for new metabolites.

Keywords:

Streptomyces , polyketide synthase , antiSMASH , genome analysis