Assessing the Synergistic Effects of Silver Nanoparticles and Nisin on Staphylococcus Aureus Genome

Due to the increased resistance of microorganisms to antibiotics and the mutagenic effects of antimicrobials, researchers are looking for safer antimicrobial agents. Silver nanoparticles have received attention due to their high antimicrobial activity, but due to their free Ag+ ions, they have mutagenic effect. The main purpose of this study was to investigate the effect of silver nanoparticles conjugated with nisin on the genome of Staphylococcus aureus. First, this bacterium is cultured in Nutrient Agar medium and after passage to Nutrient Broth, it is treated with silver nanoparticles, nisin and silver nanoparticle conjugated with nisin in ۲۵, ۵۰, ۷۵, ۱۰۰, ۱۲۵, ۱۵۰, ۲۰۰, ۳۰۰ μg/ml concentrations. Then the DNA of the control and treated samples with concentrations of ۵۰ μg/ml of silver nanoparticles, nisin and silver nanoparticles conjugated with nisin were extracted and RAPD-PCR method is used to investigate the genomic effect. The results were analyzed in NTSYS-PC software based on Dice coefficient. The results show that the inhibitory effect of silver nanoparticles conjugated with nisin on the growth of microorganisms was more than silver nanoparticles and nisin, while the genomic effect was less than nisin and silver nanoparticles. silver nanoparticles conjugated with nisin can be used as a suitable and safer antimicrobial compound for other organisms.

Keywords:

Antimicrobial , Mutagenicity , Inhibitor , RAPD-PCR , Genomic effect