Genome-wide of identification of calcium sensor gene family in Aeluropus littoralis (Gouan) Parl.

During the evolution, plants have evolved a series of complex and highly regulated signal transduction pathways to respond to adverse environmental conditions. Calcium signaling, as a vital mechanism, enables plants to respond to various external stimuli and to coordinate basic cell processes. In the present study, by comprehensive analysis of the gene family of calcium sensors in the halophyte plant A. littoralis, ۲۱ CIPK genes and ۱۶ CDPK genes were identified, and analysis of subcellular localization showed that these proteins are active in different cell compartment. The phylogenetic tree indicated that the AlCIPK and AlCDPK gene family had high homology to monocotyledon and cereals plants such as sorghum, foxtail millet and rice. Gene structure and motif composition revealed that these genes were relatively conservative in each subfamily. RNA-seq analysis of AlCIPK and AlCDPK gene family members under salinity stress in both root and shoot tissues showed that AlCDPK۵ gene had the lowest expression in both tissues, AlCDPK۲۴ and AlCDPK۲۸ had the highest expression level in shoot and root, respectively, and finally AlCIPK۱۰ and AlCIPK۱ genes had the lowest and highest expression in root and shoot, respectively. The distinctive expression patterns of AlCIPK and AlCDPK gene family members confirmed the functional and structural convergent of these genes. The findings of this study reveal the functional characteristics of the calcium gene family and provide basic information for future research on their biological functions.

Keywords:

Expression analysis , Gene family , CDPK , CIPK , halophyte