Assessment the effect of explant type and hormone on the callogenesis of Salvia tebesana Bunge

Salvia tebesana is a beneficial species among the other of Salvias in respect to its famous chemical-medicinal compounds such as tannins, monoterpenes, diterpenes, sesquiterpenes, and flavonoids. Nowadays, one of the most useful and practical ways to produce the plant secondary metabolites is tissue culture technique and callus induction. This study was based on the designation of the optimal concentration of BAP (۰, ۰.۵, and ۱ mg L-۱) and ۲,۴-D (۰, ۰.۵, ۱, and ۱.۵ mg L-۱) for callus induction from "shoot apical meristem" and "leaf" explants of S. tebesana on MS medium. The explants were isolated from S. tebesana grown in Hoagland solution. Cultured samples on MS medium were subcultured after six weeks and analyzed statistically at the end of ۸th week in terms of callus induction rate (%), form, fresh and dry weight (gr) of callus. The results showed that leaf explant was more successful than shoot apical meristem explant for callus induction. Although ۱۰۰% callus induction was observed in the "BAP۱ + ۲,۴-D۱ mg L-۱" treatment and for shoot apical meristem explant, other high callogenesis percentage was observed for leaf explants. Evaluation of callus fresh and dry weight changes also showed a significant effect (p<۰.۰۵) of the type of explants and hormones on these traits.

Keywords:

Medicinal plant , Callus culture , MS medium culture , BAP , ۲ , ۴-D