Effect of culture medium on in vitro culture of Damask rose (Rosa damascena Mill.)

Damask rose belongs to the genus Rosa and belongs to the Rosaceae family, which is one of the most important aromatic species with medicinal properties and therapeutic effects . Propagation of damask rose using conventional asexual propagation methods such as cuttings and cuttings is difficult due to the difficulty of filling the ectopic roots and its time consuming. The use of tissue culture and micropropagation techniques is an alternative method for propagation of better damask rose genotypes. In this study, in order to investigate the effect of culture medium on in vitro propagation of damask rose, two different culture media MS and WPM supplemented with ۰.۵ mg / l BAP and ۰.۱ mg / l TDZ were tested. The results of cultures showed that on shoot proliferation, MS culture medium is better to WPM, which can be attributed to the higher ionic strength of MS culture medium than WPM.

Keywords:

Tissue culture , shoot propagation , explant