Effect of explant size and culture medium on meristem culture of strawberry (Fragaria ananassa)

Nowadays, plant tissue culture is used as a useful tool for induction a new genetic variation to crops breeding as well as to produce disease-free plants. Meristem culture is one of the most important ways of producing virus-free plants. Given the importance of strawberry production and viral diseases damage in plant production, it is necessary to implement proper management strategy in the production of virus-free plant materials. This study was carried out in the plant biotechnology laboratory of Tabriz University with the aim of optimizing the meristem culture of strawberry (Fragaria ananassa) CV,Gavita and investigating the factors affecting it. In the first experiment to investigate the effect of meristematic dome size on meristem culture, three different explant including meristematic dome, meristematic dome with one primordial leave and meristematic dome with two primordial leaves separated and in cultured in MS supplemented with ۱ mg/l BAP and ۰.۲ mg/l IAA. The second experiment was conducted to investigate the effect of liquid, semi-solid and solid MS medium on meristem regeneration. All mediums supplemented with ۱ mg/l BAP and ۰.۲ mg/l IAA. All experiments were performed in a completely randomized design with five replications. Traits evaluated included percentage of survived meristem, number of regenerated shoots, and length of shoots. The results showed that there was a significant difference between the meristem dome size and the number and length of shoots obtained from meristem.The best results obtained with the meristematic dome with two leaves. Results showed that there was a significant difference between mediums in meristem induction and the best result obtained from semi-solid medium where all meristems were regenerated and produced more shoots with highest length.

Keywords:

Gavita , meristematic dome , virus , MS