Mohammad Fereidouni - Cellular and Molecular Research Center, Birjand University of Medical Sciences, Birjand, Iran & Department of Immunology, Faculty of medicine, Birjand University of Medical Sciences, Birjand, Iran.
Roya Mahdavi - Cellular and Molecular Research Center, Birjand University of Medical Sciences, Birjand, Iran
Sarah Mahmoudzade - Department of Immunology, Faculty of medicine, Birjand University of Medical Sciences, Birjand, Iran.
Hadis Rezapoor - Department of Immunology, Faculty of medicine, Birjand University of Medical Sciences, Birjand, Iran.
Alireza Fereidouni - Cellular and Molecular Research Center, Birjand University of Medical Sciences, Birjand, Iran
Afsane Bahrami - Cellular and Molecular Research Center, Birjand University of Medical Sciences, Birjand, Iran.

Background: Allergic disorders are common health problems worldwide with significant socio-economic impacts. The best diagnostic method using allergenic extract is the skin prick test. Regarding the effects of geo-climatic factors and allergenic extract source material quality, the aim of study was to determine the safety and efficacy of some in-house-developed allergenic extracts. Methods: Forty-five different allergenic extracts, including common regional pollen, foods, and dog and cat hair, as well as positive and negative extracts, were prepared from domestic sources using optimum extraction methods. All extracts passed stability and sterility testing, and sterile final products containing ۵۰% glycerin in ۱۰ and ۲۰ w/v concentrations were used. Skin prick testing was performed on volunteers and immediate or late side effects were recorded. Results: In total, ۵۶ students (mean age: ۲۱.۲±۲.۳y, M/F ratio: ۱.۰۷) participated in this study. For inhalant allergens, all extracts except dog hair extract caused positive responses. Salsola kali (Russian thistle) and Fraxinus velutina (ash tree) were the most common grass and tree pollen extracts, respectively. Of ۱۸ different food extracts, five, including egg white, tomato, fig, melon, and green pepper caused skin reactivity in only one person. No participant reported any immediate or late side effects, including large local reaction or systemic response. Conclusions: The result of the current study confirmed the safety of all our in-house-developed allergenic extracts. Regarding efficacy, almost all inhalant and five food allergens caused positive skin responses.

Allergenic extract, In-house extract, Skin prick test.