Signaling Pathway in the Osmotic Resistance Induced by Angiotensin II AT۲ Receptor Activation in Human Erythrocytes

Publish Year: 1400
نوع سند: مقاله ژورنالی
زبان: English
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JR_RBMB-10-2_018

تاریخ نمایه سازی: 10 شهریور 1400

Abstract:

Background: Angiotensin II regulates blood volume via AT۱ (AT۱R) and AT۲ (AT۲R) receptors. As cell integrity is an important feature of mature erythrocyte, we sought to evaluate, in vitro, whether Angiotensin II modulates resistance to hemolysis and the signaling pathway involved. Methods: Human blood samples were collected and hemolysis assay and angiotensin II signaling pathway profiling in erythrocytes were done. Results: Hemolysis assay created a hemolysis curve in presence of Ang II in several concentrations (۱۰-۶ M, ۱۰-۸ M, ۱۰-۱۰ M, ۱۰-۱۲ M). Angiotensin II demonstrated protective effect, both in osmotic stressed and physiological situations, by reducing hemolysis in NaCl ۰.۴% and ۰.۹%. By adding receptors antagonists (losartan, AT۱R antagonist and PD ۱۲۳۳۱۹, AT۲R antagonist) and/or signaling modulators for AMPK, Akt/PI۳K, p۳۸ and PKC we showed the protective effect was enhanced with losartan and abolished with PD ۱۲۳۳۱۹. Also, we showed activation of PKC as well as PI۳K/Akt pathways in this system. Conclusions: Briefly, Ang II protects human erythrocytes from hypo-osmotic conditions-induced hemolysis by activating AT۲ receptors and triggering both the intracellular pathways.

Authors

Camila Cristina Guimarães-Nobre

Grupo de Pesquisa em Fisiologia Eritróide - GPFisEri, Universidade Federal do Rio de Janeiro, Campus Macaé, Brazil & Programa de Pós-graduação em Endocrinologia, Faculdade de Medicina, Universidade Federal do Rio de Janeiro, Brazil.

Evelyn Mendonça-Reis

Grupo de Pesquisa em Fisiologia Eritróide - GPFisEri, Universidade Federal do Rio de Janeiro, Campus Macaé, Brazil & Programa de Pós-graduação em Endocrinologia, Faculdade de Medicina, Universidade Federal do Rio de Janeiro, Brazil.

Luana Passinho-da-Costa

Grupo de Pesquisa em Fisiologia Eritróide - GPFisEri, Universidade Federal do Rio de Janeiro, Campus Macaé, Brazil & Faculdade de Farmácia, Universidade Federal do Rio de Janeiro, Campus Macaé, Brazil.

Leandro Miranda-Alves

Laboratório de Endocrinologia Experimental- LEEx, Instituto de Ciências Biomédicas, Universidade Federal do Rio de Janeiro, Brazil & Programa de Pós-graduação em Endocrinologia, Faculdade de Medicina, Universidade Federal do Rio de Janeiro, Brazil & Progr

Clemilson Berto-Junior

Grupo de Pesquisa em Fisiologia Eritróide - GPFisEri, Universidade Federal do Rio de Janeiro, Campus Macaé, Brazil & Laboratório de Endocrinologia Experimental- LEEx, Instituto de Ciências Biomédicas, Universidade Federal do Rio de Janeiro, Brazil & Progr

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