Evaluation of SCD and FASN Gene Expression in Baluchi, Iran-Black, and Arman Sheep
Publish Year: 1395
نوع سند: مقاله ژورنالی
زبان: English
View: 227
This Paper With 7 Page And PDF Format Ready To Download
- Certificate
- من نویسنده این مقاله هستم
استخراج به نرم افزارهای پژوهشی:
شناسه ملی سند علمی:
JR_RBMB-5-1_005
تاریخ نمایه سازی: 10 شهریور 1400
Abstract:
Background: With the increasing concern for health and nutrition, dietary fat has attracted considerable attention. The composition of fatty acids in the diet is important because they are associated with major diseases including cancers, diabetes, and cardiovascular disease. The fatty acid synthase (FASN) and stearoyl-CoA desaturase (delta-۹-desaturase) (SCD) genes affect fatty acid composition (۱). The expression of SCD and FASN genes is related to an increase in conjugated linoleic acid (CLA) in dairy products, which benefits human health.The aim of current study was to investigate expression changes of SCD and FASN genes that resulted from crossbreeding the local Baluchi sheep with alien breeds.
Methods: We collected tissue samples from the mammary glands of ۲۴ single-born ewes from local Baluchi and synthetic Iran-Black and Arman sheep breeds in the Abbas Abad breeding center. After RNA extraction and cDNA synthesis, real-time PCR was performed with all samples in triplicate.
Results:The maximum and minimum expression of SCD and FASN genes was in the local Baluchi sheep and the crossbred Arman sheep, respectively.
Conclusions: With the highest SCD and FASN gene expression in local Baluchi sheep and relatively less expression of these genes in synthetic Iran-Black and Arman Sheep breeds, it may be necessary to consider the consequences of crossbreeding local sheep and the fatty acid composition of their dairy products.
Keywords:
Authors
Mohammad Salmani Izadi
Department of Animal Sciences, Ferdowsi University of Mashhad, International Campus, Mashhad, Iran
Abbas Ali Naserian
Department of Animal Sciences, Ferdowsi University of Mashhad, Mashhad, Iran.
Mohammad Reza Nasiri
Department of Animal Sciences, Ferdowsi University of Mashhad, Mashhad, Iran.
Reza Majidzadeh Heravi
Department of Animal Sciences, Ferdowsi University of Mashhad, Mashhad, Iran.
Reza Valizadeh
Department of Animal Sciences, Ferdowsi University of Mashhad, Mashhad, Iran.