Quantification of Pla or ۳, a Platanus orientalis Allergen, Grown under Different Environmental Conditions, by Sandwich ELISA

Publish Year: 1395
نوع سند: مقاله ژورنالی
زبان: English
View: 174

This Paper With 6 Page And PDF Format Ready To Download

  • Certificate
  • من نویسنده این مقاله هستم

استخراج به نرم افزارهای پژوهشی:

لینک ثابت به این Paper:

شناسه ملی سند علمی:

JR_RBMB-5-1_006

تاریخ نمایه سازی: 10 شهریور 1400

Abstract:

Background: Platanus species are widely cultured around the world and considered an important cause of allergic reactions. In the present study, we developed a sandwich ELISA to quantify Pla or ۳ allergen in P. orientalis pollen extracts grown near high-traffic roads and compared it to pollen extracts collected from rural areas as control. Methods: Pollen samples were collected from three polluted and two unpolluted sites in Mashhad, northeast Iran. Recombinant Pla or ۳ was expressed and used for polyclonal antibody production in rabbit. A sandwich ELISA was developed and validated to quantify Pla or ۳ levels in pollen extracts from the different sites. Results: The coefficients of variation (CVs) for the intra- and inter- day assays were less than ۵ and ۱۸%, respectively. The working range of the standard curve was between ۰.۱ and ۲۵ ng/ml, with the detection limit being ۰.۰۳۷ ng/ml. The recovery percentage was ۸۸-۱۰۶.۴% at working concentrations from ۰.۳۱ to ۲۶.۵ ng/ml. Pla or ۳ levels were significantly greater in pollens grown near high-traffic roads than in those grown in rural regions (p < ۰.۰۰۰۱). Conclusions: A sandwich ELISA was developed and validated to quantify Pla or ۳ in pollen extracts. Using this validated ELISA, we showed a substantial difference between the amounts of Pla or ۳ in pollens grown in different environments. This finding should be considered in developing public policies to reduce traffic pollution, which leads to reduced allergic reactions in atopic subjects.

Authors

Farnaz Sedghy

Immuno-Biochemistry lab, Immunology Research Center, Mashhad University of medical Sciences, Mashhad, Iran

Mojtaba Sankian

Immuno-Biochemistry lab, Immunology Research Center, Mashhad University of medical Sciences, Mashhad, Iran

Maliheh Moghadam

Immuno-Biochemistry lab, Immunology Research Center, Mashhad University of medical Sciences, Mashhad, Iran

Abdol-Reza Varasteh

Allergy Research Center, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran