Design and Construction of a Cloning Vector Containing the hspX Gene of Mycobacterium tuberculosis

Publish Year: 1395
نوع سند: مقاله ژورنالی
زبان: English
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شناسه ملی سند علمی:

JR_RBMB-5-1_007

تاریخ نمایه سازی: 10 شهریور 1400

Abstract:

Background: Tuberculosis (TB) is a major cause of death worldwide. Finding an effective vaccine against TB is the best way to control it. Several vaccines against this disease have been developed but none are completely protective. The aim of this study was to design and construct a cloning vector containing the Mycobacterium tuberculosis (M. tuberculosis) heat shock protein X (hspX). Methods: First, an hspX fragment was amplified by PCR and cloned into plasmid pcDNA۳.۱(+) and recombinant vector was confirmed. Results: A ۴۳۵ bp hspX fragment was isolated. The fragment was ۱۰۰% homologous with hspX of M. tuberculosis strain H۳۷Rv in GenBank. Conclusions: In this study, the cloning vector pcDNA۳.۱(+), containing a ۴۳۵-bp hspX fragment of M. tuberculosis, was constructed. This could be used as a DNA vaccine to induce immune responses in animal models in future studies.

Authors

Atieh Yaghoubi

Antimicrobial Resistance Research Center, Mashhad University of Medical Sciences, Mashhad, Iran

Ehsan Aryan

Antimicrobial Resistance Research Center, Mashhad University of Medical Sciences, Mashhad, Iran

Hosna Zare

Antimicrobial Resistance Research Center, Mashhad University of Medical Sciences, Mashhad, Iran

Shadi Alami

Antimicrobial Resistance Research Center, Mashhad University of Medical Sciences, Mashhad, Iran

Roghayeh Teimourpour

Department of Microbiology, School of Medicine, Ardabil University of Medical Sciences, Ardabil, Iran.

Zahra Meshkat

Antimicrobial Resistance Research Center, Mashhad University of Medical Sciences, Mashhad, Iran