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Title

Validity of Using Recombinant Melon Profilin, Cuc m ۲, for Diagnosis of Melon Allergy

Year: 1391
COI: JR_RBMB-1-1_003
Language: EnglishView: 35
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Authors

Mojtaba Sankian - Immunobiochemistry Lab, Immunology Research Center, Avicenna Research Institute, Mashhad University of Medical Sciences, Mashhad, Iran
Yaser Bagheri - Immunobiochemistry Lab, Immunology Research Center, Avicenna Research Institute, Mashhad University of Medical Sciences, Mashhad, Iran
Fatemeh Vahedi - Razi Vaccine & Serum Research Institute, Mashhad, Iran
Farahzad Jabbari Azad - Immunology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran
Abdol-Reza Varasteh - Immunobiochemistry Lab, Immunology Research Center, Avicenna Research Institute, Mashhad University of Medical Sciences, Mashhad, Iran

Abstract:

Background: Allergy is a clinical disorder affecting humans worldwide. Allergenic extracts prepared from natural source materials remain heterogeneous in composition and content, but are regularly used for diagnosis and immunotherapy. Recombinant allergens are suitable candidates to use in place of natural allergens; however, the recombinant allergens should be assessed and compared with the natural ones. Cuc m ۲ (profilin), one of the most important allergens of melon (Cucumis melo), has been cloned and was expressed in Escherichia coli (E. coli). We aimed to evaluate the validity of recombinant Cuc m ۲ (rCuc m ۲) in the diagnosis of melon allergy and investigate whether rCuc m ۲ could be used as a replacement for natural Cuc m ۲ (nCuc m ۲). Methods: nCuc m ۲ was purified by immuno-affinity chromatography and rCuc m ۲ was purified by metal-affinity chromatography. SDS-PAGE and western blotting were carried out to evaluate the purification methods. Skin prick tests (SPT), and enzyme immunoassays to determine specific IgE, were performed with the natural and recombinant purified allergens on ۵۳ patients with melon allergy. Results: rCuc m ۲ elicited no significantly different responses in skin compared with nCuc m ۲. All patientschr('۳۹') sera showed similar ODs in ELISAs with natural and recombinant profilin. Conclusion: rCuc m ۲ evoked strong immuno-reactivity equivalent to nCuc m ۲, and has potential for diagnosis of melon allergy.

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This Paper COI Code is JR_RBMB-1-1_003. Also You can use the following address to link to this article. This link is permanent and is used as an article registration confirmation in the Civilica reference:

https://civilica.com/doc/1263192/

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Sankian, Mojtaba and Bagheri, Yaser and Vahedi, Fatemeh and Jabbari Azad, Farahzad and Varasteh, Abdol-Reza,1391,Validity of Using Recombinant Melon Profilin, Cuc m ۲, for Diagnosis of Melon Allergy,https://civilica.com/doc/1263192

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Type of center: علوم پزشکی
Paper count: 8,097
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