Biosynthesis of organic nanocomposite using Pistacia Vera L. hull: An efficient antimicrobial agent

In this study, Pistacia Vera hull essential oil analysis was performed by GC-MS method, in which α-Pinene, D-Limonen, Isobornyl acetate compounds constitute the highest percentage of Pistacia Vera hull essential oil. Phenolic compounds are one of the most important antioxidants that are involved in various fields, including pharmacy. Pistacia Vera hull is a rich source of phenolic compounds. In this study, the most phenolic compound in Pistacia Vera hull is gallic acid and rutin, which has been identified by HPLC analysis. Here presented a quick and easy synthesis of copper nanoparticles (CuNPs). Pistachio hull extract has been used as a reducing and stabilizing agent in the preparation of CuNPs. This biosynthesis is a kind of supporter of the environment because chemical agents were not used to making nanoparticles, and on the other hand, it prevents the release of pistachio waste in nature and its adverse effects on nature. The biosynthesized CuNPs and CuNPs/silver Schiff base nanocomposite (CSS NC) were characterized by UV-VIS spectroscopy, Fourier transforms infrared spectroscopy (FT-IR), X-ray diffraction (XRD), field emission scanning electron microscopy (FE-SEM), energy-dispersive X-ray spectroscopy (EDS). CSS NC antimicrobial activity was examined by both well diffusion and determination MIC methods against four bacteria Staphylococcus aureus, Bacillus cereus, Escherichia coli, and Pseudomonas aeruginosa and two fungi Aspergillus nigero and Candida albicans. CSS NC showed significant antimicrobial activity on the samples, preventing the growth of bacteria and fungi at very low concentrations. CSS NC had the greatest effect on Escherichia coli bacteria and Aspergillus nigero fungi.

Keywords:

Nanocomposite , Biosynthesis , Antimicrobial activity , Anti fungi , Pistachio hull