zeinab behzadian - student

In the last decades, biotechnological applications have led to the optimization of the cultural conditions for the production of exopolysaccharides (EPSs) by cyanobacteria. Nostoc is a genus of cyanobacteria found in various environments that forms colonies composed of filaments of moniliform cells in a gelatinous sheath. The effect of some important nutritional and growth beside iron nanoparticles on production by Nostoc sp. was studied. Optimization of the cultural conditions was carried out using Central Composite Design (CCD), and it was achieved at pH ۸.۳۹, ۱% of NaCl and ۰.۸ g.l-۱ nitrate based in modified BG۰ medium and more than ۱۲ g.l-۱ of EPS was obtained by phenol-sulphuric acid method. The present research investigated the possibility of inducing EPS production in the presence of Fe۳O۴ nanoparticles from optimized microalga cultures. This study revealed that Nostoc sp. treatment with ۱۰ mg.l-۱ Fe۳O۴ NPs induced maximal EPS production (۴ µg.ml-۱).

Central Composite Design, Exopolysacchride, Nanoparticle, Nostoc sp, Optimization