Sleep deprivation disrupts striatal anti-apoptotic responses in ۶-hydroxy dopamine-lesioned parkinsonian rats

Objective(s): The present study was conducted to examine the effect of sleep deprivation (SD) on the anti-apoptotic pathways in Parkinsonian rats. Materials and Methods: Male Wistar rats (n = ۴۰) were assigned to four groups (۱۰ animals each): sham surgery (Sham), ۶-hydroxydopamine (۶-OHDA)-lesioned (OH), ۶-OHDA-lesioned plus grid control (OH+GC), ۶-OHDA-lesioned plus SD (OH+SD). Parkinson’s disease (PD) model was induced by the unilateral intra-striatal infusion of ۶-OHDA (۱۰ µg/rat). SD (۴ hr/day, for ۱۴ days) was induced using a multiple platforms water tank. On the last day of interventions, animals were subjected to open field test for horizontal motor performance assessment. Also, brain-derived neurotrophic factor (BDNF), Bcl-۲ and Bax were assessed in the striatum of study groups. Results: SD obscured the motor deficits of PD animals observed in open field test. BDNF level and Bcl۲/Bax ratio significantly increased in the OH group, and SD reduced their levels in the PD animals. Conclusion: SD suppressed the anti-apoptotic compensatory responses in the striatum; therefore, it may accelerate continual neuronal cell death in PD.

Keywords:

Bcl-۲ , BDNF , Bax , Sleep deprivation , Parkinson’s disease , ۶-Hydroxy dopamine