Synergistic effects of Coxsackievirus oncolytic virus in combination with copper nanoparticles, hyperthermia and radiation on CT۲۶ cell proliferation (malignant colorectal carcinoma)

Background & Aims: Today, due to changes in nutritional pattern, cancers of the gastrointestinal tract, especially the colon is one of the most common causes of death. Drug resistance of many cancers during treatment with chemotherapy drugs has led researchers to use innovative multifactorial therapies. The aim of this study was to investigate the Synergistic effects of Coxsackievirus oncolytic virus in combination with copper nanoparticles, hyperthermia and radiation on CT۲۶ cell proliferation (malignant colorectal carcinoma) as a new multifactorial method.Background & Aims: Today, due to changes in nutritional pattern, cancers of the gastrointestinal tract, especially the colon is one of the most common causes of death. Drug resistance of many cancers during treatment with chemotherapy drugs has led researchers to use innovative multifactorial therapies. The aim of this study was to investigate the Synergistic effects of Coxsackievirus oncolytic virus in combination with copper nanoparticles, hyperthermia and radiation on CT۲۶ cell proliferation (malignant colorectal carcinoma) as a new multifactorial method.Methods: In this study, after culturing CT۲۶ cells (malignant colorectal carcinoma) in ۱۳ T۲۵ flasks, they were combined with CuO nanoparticles (۱۰۰ μg/ml ،), Coxsackievirus oncolytic virus (MOI۴۰), Radiation (cGy۲۰۰) and hyperthermia (۴۱ Degrees Celsius). After determining the uptake of CuO nanoparticles to confirm the anti-cancer effects of the above factors (MTT), percentage of apoptosis, production of reactive oxygen species (ROS), release of lactatedehydrogenase (LDH), activity level Caspase ۸ and ۹ were measured. Methods: In this study, after culturing CT۲۶ cells (malignant colorectal carcinoma) in ۱۳ T۲۵ flasks, they were combined with CuO nanoparticles (۱۰۰ μg/ml ،), Coxsackievirus oncolytic virus (MOI۴۰), Radiation (cGy۲۰۰) and hyperthermia (۴۱ Degrees Celsius). After determining the uptake of CuO nanoparticles to confirm the anti-cancer effects of the above factors (MTT), percentage of apoptosis, production of reactive oxygen species (ROS), release of lactate dehydrogenase (LDH), activity level Caspase ۸ and ۹ were measured.

Keywords:

Virotherapy , Nanotherapy , Hyperthermia , Radiotherapy , CT۲۶ cell line , Colorectal malignant carcinoma