Mesenchymal stem cell’s mobility on chitosan ۲D substrates during Adipogenesis and Osteogenesis

Mesenchymal stem cells (MSCs) are multipotent cells that can differentiate into various cells, such as adipogenic and osteogenic cells.Communication with contacted materials determines the physiological activity of cells; therefore, regulating MSC fate by controlling the biophysical properties of cell contacting biomaterials is an essential issue in regenerative medicine. The stiffness of the substrate, which can be regulated by making different concentrations of the biomaterial or coating desired molecules on its surface, signals for MSCs to maintain stem cell properties or promotes differentiation. A Robust Cell attachment and mobility of the cells on the desired substrate during the differentiation are known as game-changing factors on this matter. In this study, we investigated the migration and mobility of Bone marrow-derived Stem cells in the forms of spheroids during Adipogenesis and osteogenesis (۲۱ days) on different concentrations of chitosan polymer as a ۲D cell scaffold. As an organic polysaccharide with non-toxic properties, chitosan provides desirable nano and macro grooves on its surface, which makes it a promising candidate for designing a biodegradable scaffold.Cell movement was quantified by bioimage processing and calculating the distance spheroids moved from point A to point B on each chitosan concentration every day for ۲۱ days during differentiation. Spheroid’s mobility was transiently increased early in osteogenic differentiation. At a later stage, migration was decreased, but adhesiveness was improved. The collected mobility data during Adipogenesis also showed the same pattern.

Keywords:

Mesenchymal stem cells , cell movement , chitosan , diffrentiation