Exposed loops of BauA and ZnuD provide immunity protection against Acinetobacter baumannii in mice sepsis model

Publish Year: 1400
نوع سند: مقاله کنفرانسی
زبان: English
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شناسه ملی سند علمی:

MEDISM22_022

تاریخ نمایه سازی: 8 مهر 1400

Abstract:

Background and Aim : Acinetobacter baumannii causes severe nosocomial infections and is a difficult-to-treat pathogen due to the development of multidrug-resistant strains. Vaccines represent an alternative promising strategy for the control of infections caused by A. baumannii. Methods : Antibody therapy may be an alternative option for treatment of infections caused by the multi-drug resistant (MDR) A. baumannii. As this pathogen possesses multiple capsular serotypes, a universal antibody therapy would need to target conserved protein antigens rather than the capsular polysaccharides. In this study, we targeted iron and zinc acquisition systems by selecting immunologically active loops from Baumannii acinetobactin Utilization A (BauA) and Zinc uptake component D (ZnuD) in A.baumannii. The surface epitopes were displayed and a hybrid antigen from the loopless C lobe of the TbpB protein of Neisseria meningitides referred to as LCL was constructed. The nucleotide sequences of the immunogenic loops of BauA and ZnuD were amplified and implanted in the LCL. The recombinant genes were expressed and purified. The purified proteins were administered to BALB/c mice. Both active and passive immunizations were carried out. The mice were then challenged with a clinical isolate of A.baumannii.Results : Indirect ELISA confirmed significant antibody rise to the antigens. In the active immunization, the survival rates of ۴۲.۸%, ۱۴%, and ۴۲.۸% were achieved with the loops derived from BauA, ZnuD, and combination of both loops respectively. Significant decrease in the bacterial loads were noted in the spleen, liver and lungs of the immunized mice groups. Conclusion : Loop ۷ of BauA plays an effective role in immunoprotectivity against A.baumannii infections.

Keywords:

Acinetobacter baumannii- BauA- ZnuD- Immunogenicity- Siderophore- Vaccine

Authors

Mahdi Pishgahi

Department of Biology, Shahed University, Tehran-Iran

Iraj Rasooli

Molecular Microbiology Research Center and Department of Biology, Shahed University, Tehran-Iran

Somshukla Chaudhuri

Department of Microbiology, Immunology and Infectious Diseases, Cumming School of Medicine, University of Calgary, Canada

Vahid Farshchi Andisi

Department of Microbiology, Immunology and Infectious Diseases, Cumming School of Medicine, University of Calgary, Canada

Abolfazl Jahangiri

Applied Microbiology Research Center, Systems biology and poisonings Institute, Baqiyatallah University of Medical Sciences, Tehran, Iran.

Anthony B. Schryvers

Department of Microbiology, Immunology and Infectious Diseases, Cumming School of Medicine, University of Calgary, Canada