Expression of bap Gene in Acinetobacter baumannii by Real-Time PCR assay from Clinical Specimens in Khorramabad, Iran

Background and Aim : Biofilm-associated protein (Bap) in Acinetobacter baumannii is a major factor in biofilm production and persistence in the hospital environment. Therefore, the aim of this study was detection of bap gene in Acinetobacter baumannii by Real-Time PCR from Clinical Specimens in Khorramabad, Iran Methods : In this cross-sectional study from April ۲۰۱۷ to April ۲۰۱۸, A. baumannii strains from clinical samples were collected and identified by microbiological and biochemical tests. Expression of Bap gene was evaluated by Real-Time PCR method. Data were analyzed with statistical package for the social science (SPSS) version ۲۴. Results : In our study ۴۳ A. baumannii isolated from teaching hospitals in Khorramabad, Iran. Out of them, ۲۳ sample isolated from phlegm, ۸ sample isolated from wounds, ۳ sample isolated from urine, ۳ sample isolated from tissue, ۳ sample isolated from blood, and۳ sample isolated from chest sputum. According to PCR results all isolates had Bap gene, except one isolate. Real-time PCR results of the expression of bap gene showed significant difference among A. baumannii isolated from different clinical sample.Conclusion : The expression of bap gene in phlegm and wound samples showed the highest value and had a significant difference compared to other samples (P <۰.۰۰۰۱).

Keywords:

Acinetobacter baumannii , Biofilm-associated protein (Bap) , Real-time PCR