Invitro investigation of the effect of Aztreonam and Ceftazidime on NDM producer Klebsiella pneumonia isolates.

Background and Aim : NDM is novel board-spectrum Metallo B lactamase with the capability to grant resistance to almost B- lactam antibiotics. Its widespread dissemination made treatment options a major challenge to combat, causing threat to public health worldwide. Due to antibiotic resistance problems, development of effective therapeutics for infections caused by NDM producing strains is urgently required. The present work was aimed to assess the effectiveness of current CPE detection guidelines we analyzed the Aztreonam and Ceftazidime MIC distribution for NDM producer Klebsiella pneumonia isolates. Methods : THIS study was conducted in ۲۰۲۱ under the supervision of Arak University of Medical Sciences. Samples used in this study are from a surveillance study with doi: ۱۰.۱۱۸۶/s۱۲۹۴۱-۰۲۰-۰۰۳۴۹-z. ۱۲۲ Klebsiella spp. isolates were cultured from extraintestinal specimens such as blood, urine , etc of patients admitted to the tertiary referral hospital in Semnan that ۱۶ of them were NDM positive. The microdilution broth method was performed to analyze the minimal Inhibitory Concentration Results : In this study, in ۱۶ NDM producer Klebsiella pneumonia appeared the susceptibility to Aztreonam in ۱۲ isolates were ۶۴mg/ml and in ۴ isolates were ۳۲mg/ml and susceptibility to Ceftazidime in ۱۱ isolates were ۱۲۸mg/ml and in ۵ isolated were ۶۴mg/ml. To determine microbial resistance, MIC method was used on NDM samples, while in some studies, disk diffusion method was used. It seems that the use of microdilution method to determine the MIC leads to more accurate results than the E-test method.Conclusion : Given paucity to treatment options there is a critical need to stop the spread of CPE in laboratory setting has been challenging. This study indicated Aztreonam is more effective antibiotic than Ceftazidime. A synergistic effect may be observed due to the combination of these two antibiotics that I will consider in future research this.

Keywords:

Ceftazidime , Aztreonam , Klebsiella pneumonia , Microbial resistance , New Delhi Metallo-β-lactamase