High frequency of genes KPC in Klebsiella pneumoniae strains isolated from clinical samples of a tertiary hospital

Background and Aim : Due to the increasing prevalence of antibiotic resistance to beta-lactam and Carbapenem producing bacteria, Identification of the gene isolates of these enzymes is essential for the timely treatment of such isolates as KPC-type Klebsiella strains. Therefore, the aim of this study was to identify and investigate the frequency of Klebsiella pneumonia strains producing KPC carbapenemase in clinical samples from Valiasr Hospital Arak, IranMethods : In this cross-sectional study, ۱۰۰ samples of Klebsiella pneumoniae were isolated from clinical samples of Valiasr Hospital. After confirmation by biochemical tests, the susceptibility of all isolates to imipenem was determined by disk diffusion method Then, carbapenemase inhibitory method(CIM) method was performed to evaluate the carbapenemase resistance and to confirm the MIC by E-test. To confirm the metallo-beta-lactamase resistance, the combined disk test (CDDT) IMP / EDTA was performed. Finally, using PCR method, blaKPC gene was investigated in Klebsiella strains with carbapenemase resistanceResults : In this study, out of ۱۰۰ strains of Klebsiella pneumoniae, ۸۴ (۸۴%) samples were identified as Carbapenem resistance by disk test and MIC method. Out of ۸۴ isolates ۳۰ (۳۶%) were carbapenemase resistance and ۵۴(۶۴%) metallo-beta-lactamase resistance were observed. Among isolates with carbapenemase resistance, all samples carried the blaKPC gene.Conclusion : The results of the present study showed that Klebsiella strains isolated from clinical samples of Valiasr Hospital Arak are associated with to carbapenems resistance along with the expression of blaKPC gene. Because the present gene can be spread among bacteria through genetically engineered elements, it is a serious warning sign in the treatment of infections caused by Klebsiella pneumoniae.

Keywords:

Klebsiella pneumoniae , carbapenemase Resistance , KPC gene