Isolation and in vitro evaluation bacteriolytic effect on bacteriophages against reference multi Drug-resistant Gram-negetive and Gram-positive strains from sewage sample

Publish Year: 1400
نوع سند: مقاله کنفرانسی
زبان: English
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MEDISM22_194

تاریخ نمایه سازی: 8 مهر 1400

Abstract:

Background and Aim : The spread of multidrug resistance in pathogenic bacteria has become one of the most important discussions among physicians and Multidrug-resistant bacteria account for a high percentage of annual mortality. Multidrug resistance is common in both Gram-positive and Gram-negative bacteria. Meticillin Resistant Staphylococcus aureus is one of the multidrug resistant bacteria. Resistant in Klebsiella pneumonia and Escherichia coli is more due to production of extended resistant beta lactamas. Phagetherapy is a therapeutic Method for drug resistant, which use of viruses that do not have infection for human. The aim of this study was to isolate and evaluation effect bacteriophages against reference multi Drug-resistant Gram-negetive and Gram-positive strains from sewage sample. Methods : After isolation bacteriophage from sewage wastewater, reference strains Meticillin Resistant Staphylococcus aureus, Klebsiella pneumonia and Escherichia coli with Extended Resistant Beta lactamas from Tehran Institute of Pasteur prerared and their specific bacteriophages were identified. For see phage plaque use double layer agar method and then for evaluation bacteriolytic effect use one-layer agar method, the Finally, for study the morphology of bacteriophages was used scanning electron microscopy.Results : Formation clear plaque in the method of double layer agar for Escherichia coli, klebsiella pneumonia and Staphylococcus aureus indicated cell lysis by bacteriophages. In addition, the morphology of this bacteriophage was identified by TEM.Conclusion : The results show that bacteriophages are specific and according increasing resistance Phage therapy can open a new view for multi-drug resistant bacteria as a new treatment.

Authors

Vahideh Fanaee

Cellular and Molecular Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, Iran.

Majid validi

Cellular and Molecular Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, Iran.

behnam zamanzad

Cellular and Molecular Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, Iran.

ali karimi

Cellular and Molecular Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, Iran.