Evaluation of antibacterial and anti-biofilm effect of zinc selenide dual nanoparticles by aqueous extract of mountain tea extract (Stachys lavandulifolia) against pathogenic clinical isolates

Background and Aim : Today, there are various methods for the synthesis of nanoparticles, green nanoparticle center is one of the popular methods, which due to its simplicity and low cost has been considered by many researchers in biology and medicine. Zinc selenide nanoparticles (NPznse) have many applications in biomedicine, but so far its various biological aspects have not been studied in detail.Methods : In this study, dual nanoparticles of zinc selenide were synthesized by aqueous extract of mountain tea extract and confirmed by UV-Vis, FT-IR, EDX and SEM and TEM electron microscopy. The antibacterial and antibiofilm properties of the synthesized nanoparticles were determined quantitatively and qualitatively in Pseudomonas aeruginosa, Escherichia coli, and Staphylococcus aureus using agar well dilution method and ۹۶ microplate method.Results : Zinc selenide dual nanoparticles were synthesized by aqueous extract of mountain tea, the synthesis of which was confirmed by changing its color to light brown. Analysis of this nanoparticle by spectrophotometer showed the absorption peak in the region of ۳۰۵ nm. These nanoparticles had a spherical structure with dimensions of about ۳۰-۶۰ nm. The diameter of the antibacterial activity halos of zinc dual selenide nanoparticles was between ۹.۵-۱۷ mm. Also, these nanoparticles at a concentration of ۱۰۰ ppm had good anti-biofilm activity in the target bacteria.Conclusion : Based on the results obtained in this study, dual nanoparticles of zinc selenide by aqueous extract of mountain tea extract can be used as an effective antimicrobial agent to inhibit the biofilm formation of Pseudomonas aeruginosa, Staphylococcus aureus, and Escherichia coli.

Keywords:

Biofilm , Antibacterial , Zinc selenide dual nanoparticles