Antibacterial Activity of Zinc Oxide Nanoparticles Synthesized by Cinnamomum zeylanicum Bark Extract

Background and Aim : Recently, the production of nanoparticles is one of the most successful hopes in the treatment of bacterial infections. The aim of this study was to investigate the antimicrobial effect of Zinc oxide nanoparticles synthesized by Cinnamomum zeylanicum bark extract.Methods : In this study, biosynthesis of zinc oxide nanoparticles from C. zeylanicum was performed using precursor zinc acetate dehydrates and NaOH. The nanoparticles produced were characterized by UV-vis spectroscopy, X-ray diffraction (XRD) spectroscopy, Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), and energy-dispersive X-ray spectroscopy (EDX). Then, the antimicrobial activity of synthesized nanoparticles was investigated by broth microdilution method against two strains of gram-positive bacteria Staphylococcus aureus, Bacillus subtilis, and two strains of gram-negative bacteria Pseudomonas aeruginosa, Escherichia coli. Results : The results showed that the zinc oxide nanoparticles were synthesized in a polygonal to round shape with an average size of ۳۷ nm. The determining minimum inhibitory concentration (MIC) of zinc oxide nanoparticles against the studied bacteria was reported between ۳.۱۲۵ - ۱۲.۵ μg/ml. Conclusion : According to the results, ZnO nanoparticles synthesized using C. zeylanicum bark extract have antibacterial effects.

Keywords:

Antimicrobial activity , Green Synthesis , Cinnamomum zeylanicum Extract , Zinc Oxide Nanoparticles.