Biosynthesis of iron nanoparticles and evaluation of its antimicrobial activity by Bacillus cereus

Background and Aim : The chemical methods used to synthesize nanoparticles mainly lead to environmental pollution, instead of leaving some toxic reagents and not using the resulting nanoparticles in biological applications. Due to the environmental pollution caused by the synthesis of nanoparticles by chemical and physical methods and also the incompatibility of their use in medicine, the purpose of this study is to synthesize iron nanoparticles and investigate its antibacterial activity.Methods : In this study, Bacillus cereus was cultured in broth nutrient medium. Then active bacterial suspension in a ratio of ۱: ۱ with a solution of ۰.۱ M Iron nitrate synthesis and biosynthesis of iron nanoparticles were performed after ۲۲ minutes at room temperature. Nanoparticle synthesis was confirmed by spectrophotometer, electron microscopy and X-ray diffraction. Then, the antimicrobial effects of nanoparticles made using disk diffusion and tubular dilution methods on standard strains of Staphylococcus aureus, Bacillus cereus, Pseudomonas aeruginosa were evaluated. Results : The absorption peak of iron nanoparticles was observed at wavelengths of ۲۲۰ nm. The synthesized iron nanoparticles were multifaceted. The average size of iron nanoparticles was in the range of ۵۰-۷۰ nm. For Staphylococcus aureus, Bacillus cereus, Pseudomonas aeruginosa were measured as ۰.۰۱۲, ۰.۰۰۶۹, ۰.۰۱۷, ml / mg, respectively.Conclusion : The results showed that the synthesized nanoparticles have antibacterial properties against pathogenic strains. The highest diameter of growth inhibition halo was observed by iron nitrate bioparticles for Pseudomonas aerosinosa and the lowest diameter of growth inhibition zone was observed for Bacillus cereus.

Keywords:

Bacillus cereus , Antibacterial activity , Iron nanoparticles