Evaluation of mPEG-PLA nanoparticles as vaccine delivery system for modified protective antigen of Bacillus anthracis

Publish Year: 1400
نوع سند: مقاله ژورنالی
زبان: English
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JR_NAMJ-8-4_005

تاریخ نمایه سازی: 11 مهر 1400

Abstract:

Objective(s): Bacillus anthracis is the cause of the fatal anthrax. Available anthrax vaccines have low stability and require multiple injections in order to be effective. Poly lactic acid (PLA) has been approved as a biodegradable and biocompatible polymer for drug and vaccine delivery applications. The purpose of this study is to evaluate the antibody titer against the protective antigen recombinant protein (PA۶۳) encapsulated by the mPEG-PLA double-block copolymers and to compare with the non-encapsulated PA۶۳.Materials and Methods: To attain this purpose, to start, the desired protein was purified and confirmed and then PA۶۳ was encapsulated with mPEG-PLA double-block copolymers using a water- oil- water solvent evaporation method. Produced nanoparticles was characterized in terms of morphological specifications using scanning electron microscopy, size and polydispersity index using dynamic light scattering and zeta potential using a zeta seizer. The synthesized nanoparticle antigenic content and also its antigen release profile was measured. In the following, the nanoparticles containing antigens (PA۶۳-NPs), blank nanoparticles (mPEG-PLA- NPs), PA۶۳ and adjuvant control were injected subcutaneously to mice and the IgG polyclonal antibody titr was measured by indirect ELISA. Finally to evaluate biocompatibility and toxicity, synthesized nanoparticles were investigated  by cell culture testing.Results:  The results of this study showed that the synthesized nanoparticles are of good quality. ELISA results showed that antibody production titr in mice receiving PA۶۳-NPs was higher than those receiving the PA۶۳ (P<۰.۰۵). Cell culture results revealed that the synthesized nanoparticles have no toxicity.Conclusion: The findings of the study indicated that the obtained nano vaccine formulations had a higher ability than non-encapsulated recombinant proteins to stimulate the immune system of animal, and that PLA could be used as an appropriate carrier for an effective, stable, safe and biodegradable engineered recombinant vaccine against anthrax.

Authors

Seyed Masih Etemad aubi

Department of Biology, Faculty of Basic Science, Imam Hossein University, Tehran, Iran

Hosein Honari

Department of Biology, Faculty of Basic Science, Imam Hossein University, Tehran, Iran

Hamed Bagheri

Faculty of Interdisciplinary Science and Technology, Tarbiat Modares University, Tehran, ۱۴۱۱۵-۳۳۶, Iran

Rohollah Ghasemi

Department of Nanobiotechnology, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, ۱۴۱۱۵-۱۷۵, Iran

Mojtaba Noofeli

Department of Human Bacterial Vaccines Production & Research, Razi Vaccine and Serum Research Institute, Karaj, Agricultural Research, Education and Extension Organization (AREEO), Tehran, Iran

Seyed Mojtaba Aghaie

Department of Biology, Faculty of Basic Science, Imam Hossein University, Tehran, Iran

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