Effects of synthetic silymarin-PLGA nanoparticles on M۲ polarization and inflammatory cytokines in LPS-treated murine peritoneal macrophages

Publish Year: 1400
نوع سند: مقاله ژورنالی
زبان: English
View: 183

This Paper With 9 Page And PDF Format Ready To Download

  • Certificate
  • من نویسنده این مقاله هستم

استخراج به نرم افزارهای پژوهشی:

لینک ثابت به این Paper:

شناسه ملی سند علمی:

JR_IJBMS-24-10_016

تاریخ نمایه سازی: 11 مهر 1400

Abstract:

Objective(s): Silymarin (SM) is a natural antioxidant compound with good anti-inflammatory effects, but its poor water solubility restricts its usage. Today, nanomaterial compounds (such as PLGA Poly D, L-lactic-co-glycolic acid) can provide a proper drug delivery system and help improve the accessibility of bioactive compounds to cells and tissues. Materials and Methods: In this study, PLGA nanoparticles (NPs) containing SM (SM-PLGA) were synthesized and characterized and their biological effects were evaluated on M۲ macrophage polarization to regulate inflammation. SM-PLGA NPs were fabricated by the oil in water emulsion (O/W) method. Macrophages (MQs) were isolated from mouse peritoneum by the cold RPMI lavage protocol. Primary mouse MQ cells were treated by SM and SM-PLGA NPs and then stimulated with lipopolysaccharide (LPS). M۲ polarization was evaluated by measurements of cytokine secretion levels (TNF-α, IL۱-β, and IL-۱۰), flow cytometry markers (F۴/۸۰, CD۱۱b, CD۳۸, and CD۲۰۶), and the expression of specific proteins (M۲ Ym۱ and Fizz۱).Results: SM-PLGA characterization showed that NPs were fabricated in the desired form. SM and SM-PLGA decreased pro-inflammatory cytokines (TNF-α and IL۱-β) and increased IL۱۰ as an anti-inflammatory cytokine. On the other hand, the M۲-associated markers and proteins increased following treatment with SM and SM-PLGA. Post-hoc analysis indicated that these changes were more pronounced in the SM-PLGA group.Conclusion: This study revealed that SM-PLGA could markedly promote M۲ polarization, thereby providing a valuable medical approach against sepsis and septic shock.

Authors

Mojgan Azadpour

Research Center of Pediatric Infectious Diseases, Institute of Immunology and Infectious Diseases, Iran University of Medical Sciences, Tehran, Iran

Mohammad Morad Farajollahi

Department of Medical Biotechnology, School of Allied Medical Sciences, Iran University of Medical Sciences,Tehran, Iran

Hassan Dariushnejad

Department of Medical Biotechnology, Faculty of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran

Ali Mohammad Varzi

Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran

Amir Varezardi

Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran

Mitra Barati

Research Center of Pediatric Infectious Diseases, Institute of Immunology and Infectious Diseases, Iran University of Medical Sciences, Tehran, Iran

مراجع و منابع این Paper:

لیست زیر مراجع و منابع استفاده شده در این Paper را نمایش می دهد. این مراجع به صورت کاملا ماشینی و بر اساس هوش مصنوعی استخراج شده اند و لذا ممکن است دارای اشکالاتی باشند که به مرور زمان دقت استخراج این محتوا افزایش می یابد. مراجعی که مقالات مربوط به آنها در سیویلیکا نمایه شده و پیدا شده اند، به خود Paper لینک شده اند :
  • Liu Y-C, Zou X-B, Chai Y-F, Yao Y-M. Macrophage polarization ...
  • Saito H, Timurkaynak F, Borzykowski T, Kilpatrick C, Pires D, ...
  • Rhodes A, Evans LE, Alhazzani W, Levy MM, Antonelli M, ...
  • Wang N, Liang H, Zen K. Molecular mechanisms that influence ...
  • Hamilton JA, Tak PP. The dynamics of macrophage lineage populations ...
  • Zhu Y, Fan S, Lu Y, Wei Y, Tang J, ...
  • Saiyyad, I.M., Bhambere, D.S., Kshirsaga, Dr.S., Formulation and optimization of ...
  • Karimi G, Vahabzadeh M, Lari P, Rashedinia M, Moshiri M, ...
  • Dasgupta A. Antiinflammatory Herbal Supplements. Chapter ۴ Translational Inflammation ۲۰۱۹; ...
  • Kim EJ, Lee MY, Jeon YJ. Silymarin inhibits morphological changes ...
  • Shi J, Votruba AR, Farokhzad OC, Langer R, Nanotechnology in ...
  • Siddiqui K, Waris A, Akber H, Munir K, Mir M, ...
  • Kızılbey K. Optimization of Rutin-Loaded PLGA Nanoparticles Synthesized by Single-Emulsion ...
  • Javed S, Kohli K, Ali M. Reassessing bioavailability of silymarin. ...
  • Mohammadian F, Abhari A, Dariushnejad H, Zarghami F, Nikanfar A, ...
  • El-Sherbiny I M, Abdel-Mogib M, Dawidar A-A M, Elsayed A, ...
  • Mohamed S.El-SamaligyNagia N.AfifiEnas A.Mahmoud, Evaluation of hybrid liposomes-encapsulated silymarin regarding ...
  • Gonçalves, Ricardo, and David M. Mosser. The isolation and characterization ...
  • Rusmana D, Elisabeth M, Widowati W, Fauziah N, Maesaroh M. ...
  • Hashimoto D, Miller J, Merad M. Dendritic cell and macrophage ...
  • Lee S-H, Lee S-M. Suppression of hepatic cytochrome p۴۵۰-mediated drug ...
  • Mehta A, Brewington R, Chatterji M, Zoubine M, Kinasewitz GT, ...
  • Gazzinelli RT, Wysocka M, Hieny S, Scharton-Kersten T, Cheever A, ...
  • Namangala B, De Baetselier P, Brijs L, Stijlemans B, Noël ...
  • Namangala B, Noël W, De Baetselier P, Brys L, Beschin ...
  • Hunter CA, Ellis-Neyes LA, Slifer T, Kanaly S, Grünig G, ...
  • Goerdt S, Orfanos CE. Other functions, other genes: alternative activation ...
  • Han MS, Jung DY, Morel C, Lakhani SA, Kim JK, ...
  • Arranz A, Doxaki C, Vergadi E, Torre YM, Vaporidi K, ...
  • Mandal D, Fu P, Levine AD. Redox regulation of IL-۱۳ ...
  • Jablonski KA, Amici SA, Webb LM, de Dios Ruiz-Rosado J, ...
  • Ulloa L, Tracey KJ. The ‘cytokine profile’: a code for ...
  • Kang JS, Jeon YJ, Park S-K, Yang K-H, Kim H-M. ...
  • CİHAN B, YARAT A, AKBAY TT, ŞENER G. Investigation of ...
  • Lim R, Morwood CJ, Barker G, Lappas M. Lim R, ...
  • Liu YC, Yao FH, Chai YF, Dong N, Sheng ZY, ...
  • Asghar Z, Masood Z. Evaluation of antioxidant properties of silymarin ...
  • Kiruthiga PV, Shafreen RB, Pandian KS, Arun S, Govindu S, ...
  • Kim SH, Oh DS, Oh JY, Son TG, Yuk DY, ...
  • Snima K, Arunkumar P, Jayakumar R, Lakshmanan V-K. Silymarin encapsulated ...
  • Chu HX, Broughton BR, Ah Kim H, Lee S, Drummond ...
  • Hume DA. Macrophages as APC and the dendritic cell myth. ...
  • Shiratori H, Feinweber C, Luckhardt S, Wallner N, Geisslinger G, ...
  • Juma KM, Ahmed ZA, Numan IT, Hussain SAR. Dose-dependent anti-inflammatory ...
  • Polyak SJ, Morishima C, Shuhart MC, Wang CC, Liu Y, ...
  • Martinez FO, Gordon S. The M۱ and M۲ paradigm of ...
  • Fiorentino DF, Bond MW, Mosmann TR. Two types of mouse ...
  • Kim T-H, Yang K, Kim M, Kim H-S, Kang JL. ...
  • Tong W, Zhang C, Hong T, Liu D, Wang C, ...
  • نمایش کامل مراجع