Zhao Wang - Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Technology and Business University, Beijing ۱۰۰۰۴۸, China
Yang Liu - Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Technology and Business University, Beijing ۱۰۰۰۴۸, China
Chen Fan - Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Technology and Business University, Beijing ۱۰۰۰۴۸, China
Xueli Cao - Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Technology and Business University, Beijing ۱۰۰۰۴۸, China

Background: Sophora japonica L. (Leguminosae), a well-known traditional medicine in Asia, is offcially listed in the Chinese and European Pharmacopoeia. Its buds and fruits have been used as medicinal agents with signifcant biological activity. Pharmacologic studies and clinical practice have demonstrated that they have benefcial effects in the treatment of many diseases. Herein, we developed a simpler and effective separation method of flavones and isoflavones from S. japonica fruit by one-step HSCCC based on the COSMO-RS model.Methods: HSCCC was applied for the preparative separation and purifcation of fve active compounds from S. japonica L. fruit (three flavone glycosides and two isoflavone glycosides). Under the assistance of a COSMO-RS, one-step separation with the two-elution solvent system that was designed.Results: The frst elution was conducted with the lower phase of ethyl acetate-n-butanol-water (۵:۱:۵, v/v), and the second elution was performed with the same one containing ۴.۰ mmol L-۱ of NH۳·H۲O. From ۲۰۰ mg of crude extract, ۱۱.۲ mg of kaempferol-۳-O sophoroside (I), ۳.۲ mg of rutin (II), ۳.۴ mg of kaempferol-۳-O-α-L-ramnopyranosyl-(۱–۶)-β-Dglucopyranoside (III), ۱۱.۵ mg of sophorabioside (IV), and ۱۰.۱ mg of sophoricoside (V) were obtained. Their purity values were ۹۸.۱%, ۹۷.۶%, ۹۹.۵%, ۹۹.۲%, and ۹۸.۳%, respectively.Conclusion: The fve targets of I-V can be used as reference substances for chromatographic purposes as well as for the further physiological studies. The developed method is instructive for the separation of other flavonoids too.

Flavonoid, Glycosides, Sophora japonica, Counter-current chromatography