Development of a Label-free MOF-based Genosensor for Sensitive Determination of Haemophilus Influenzae in Human Plasma Samples

In this research work, an innovative label-free DNA genosensing assay based on a direct hybridization followed by DPASV in the presence of [Fe(CN)۶]۳-/۴- was developed for recognizing H. Influenza genome in human plasma samples. To attain this objective, Zn based MOF was synthesized and combined with carboxymethyl cellulose (CMC), which were immobilized on the surface of Au electrode and AuNPs were immobilized on the Zn based MOF/CMC/Au modified electrode surface. Well-fabricated genosensing bio-assay represented high specificity, sensitivity and good performance for determination of FUCK gene from Haemophilus Influenza genome. Under optimal circumstances, LOD and LOQ were acquired ۱.۴۸ fM and ۳.۲۳ fM. The fabricated biosensing assay presented high selective ability of one, two and three-base mismatched sequences. In addition, negative control of the genosensing assay for investigation of the selectivity was performed. Likewise, reproducibility and repeatability of the related bio-assay was investigated as well. It is to be noted that the organized genosensing bio-assay can be straightforwardly reused and regenerated to assess hybridization process.

Keywords:

Haemophilus Influenza , Fastidious bacteria , Genosensor , DNA hybridization , MOF , AuNPs