Houra Nekounam - Department of Medical Nanotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran
Rassoul Dinarvand - Department of Pharmaceutics, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
Rahele Khademi - International affairs, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran
Fatemeh Asghari - Department of Medical Nanotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran
Narges Mahmoodi - Sina Trauma and Surgery Research Center, Tehran University of Medical Sciences, Tehran, Iran
Hossein Arzani - Department of Medical Nanotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran
Elham Hasanzadeh - Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Mazandaran University of Medical Sciences, Sari, Iran
Amin Hadi - Cellular and Molecular Research Center, Yasuj University of Medical Sciences, Yasuj, Iran
Roya Karimi - Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran
Morteza Kamali - Department of Medical Nanotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran
Masood Khosravani - Department of Medical Nanotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran

Indirubin can be applied as an anti-cancer drug for inhibition of brain tumors. However, its performance is reduced due to hydrophobicity. In this study, we synthesized cationic human serum albumin (CHSA) nanoparticle by a new hybrid approach (with chemical and mechanical base) for improvement the surface chemistry of albumin and the amount of indirubin loaded CHSA nanoparticle. In this study, the generated mechanical force from a high-pressure homogenizer (HPH) was used to make nanoparticles with a certain size with narrow polydispersity. The results indicated that the size of indirubin loaded CHSA nanoparticles were ۱۳۰ nm and their zeta potential were +۹. Besides, the encapsulation efficiency and drug loading capacity were found to be ۸۵% and ۵.۸ %, respectively. To the best to our knowledge, this is the first time that indirubin has been used in albumin nanoparticles. In this study, indirubin loaded CHSA nanoparticles was shown can be a potential candidate for drug delivery in the treatment of glioblastoma. Moreover, the cationized form allows the chemical agent to be transmitted to the brain.

Brain tumor, Cationated Human Serum Albumin, Indirubin, Glioblastoma, Protein Nanoparticles, sustained release