Preparation and In Vitro Characterization of Alginate Microspheres Encapsulated with Autoclaved Leishmania major (ALM) and CpG -ODN
Publish place: Iranian Journal of Basic Medical Sciences، Vol: 10، Issue: 2
Publish Year: 1386
نوع سند: مقاله ژورنالی
زبان: English
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شناسه ملی سند علمی:
JR_IJBMS-10-2_002
تاریخ نمایه سازی: 27 مهر 1400
Abstract:
Objective The goal of this study was to prepare and characterize alginate microspheres as an antigen delivery system and adjuvant for immunization against leishmaniasis. Materials and Methods Microspheres were prepared by an emulsification technique and characterized for size, encapsulation efficiency, and release profile of encapsulates. Selection of appropriate parameters (viscosity of alginate, emulsifier, and sonication times) enabled the preparation of alginate microspheres with a mean diameter of ۱.۸ ± ۱.۰μm, as determined by Scanning Electron Microscopy and Particle Size Analyzer. Results The encapsulation efficiency was about ۳۴.۲ ± ۶.۷% for autoclaved leishmania major and ۶۳.۵ ± ۶.۹% for CpG-ODN, as determined by spectrophotometric assays. In vitro release profile showed a slow release rate for encapsulated ALM, while higher release rate was observed for CpG-ODN. The molecular weight was evaluated by SDS-PAGE and showed that the process of encapsulation did not affect the molecular weight of the entrapped antigen. Conclusion With regard to the optimum diameter (less than ۵ μm), slow release rate and preservation of antigen
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Authors
Z. Ghiasi
School of Pharmacy, Mashhad University of Medical Sciences, P.O. Box ۹۱۷۷۵-۱۳۶۵, Mashhad, Iran
S. A. Sajadi Tabasi
School of Pharmacy and Pharmacological Research Center of Medicinal Plants, Mashhad University of Medical Sciences, Mashhad, Iran
M. Tafaghodi
School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
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