New p۵۳ Gene Mutation in non-Cancerous Mustard Gas Exposed Lung

Objective Mustard gas (MG) is a poisoning chemical, mutagenic and carcinogenic alkylating agent. It is used during World War I and also Iran-Iraq conflict. The p۵۳ tumor suppressor gene is involved in the pathogenesis of malignant disease. The aim of this study is to determine possible mutation in p۵۳ gene of lung sample from mustard gas exposed patients. Material and Methods Twelve lung biopsy samples from ۱۲ Mustard Gas exposed soldiers cases along with control cell line were studied for the presence of mutations in exons ۴-۹ of the p۵۳ gene by PCR and direct sequencing. Results Among examined biopsies most of the samples demonstrated normal polymorphism with no significant defected mutations but in one sample one type of p۵۳ gene alteration at codon ۲۷۸ (CCT^CCA) on transcribed strand was detected. This Mutation has not been observed in another studies related to mustard gas exposure and p۵۳ mutation databases. Conclusion In this study we have reported for the first time new p۵۳ mutation in the lung sample of MG exposed patients. It is concluded that only one silent mutation were scanned with no signs of any type of cancer. This type of mutation was not in IARC p۵۳ gene mutation database. Moreover, surrounding sequences of the mutated p۵۳ gene codons have more ۵'-GT and ۵-GC sequences which have been found both by our study and only one another study on Japanese exposed to MG.

Keywords:

Lung Biopsy , Mustard Gas , p۵۳ mutation , PCR , Sequencing