A Morphometeric Study on CA۳ Hippocampal Field in Young Rats Following Maternal Administration of Boswellia Serrata Resin During Gestation

Objective It has previously been shown that prenatal maternal administration of Boswellia serrata gum resin (Frankincense) improved learning and memory performance associated with an increase in the size of neuronal bodies in CA۳ (Cornu Ammonis) of hippocampus. Continuing the previous work, a morphometric study was designed on CA۳ field to examine precisely the effect of prenatal administration of frankincense on the structure of this region. Materials and Methods ۲ months-old male Wistar rats whose mothers were given orally the aqueous extract of the Boswellia serrata (۰.۱ g/kg/day) during gestation (۳ weeks) were anesthetized and transcardially perfused with phosphate-buffered solution of ۴% formaldehyde and ۱% glutaraldehyde (n=۸). Each brain was removed and divided into two hemispheres. One hemisphere selected at random for estimating the volumes of CA۳ layers, and the other for morphometeric analysis of CA۳ neuronal dendrites. The Cavalieri principle employed to estimate the volumes and a quantitative Golgi study used to analyse the dendritic arborizations. Results Comparisons revealed that the control rats had lower volumes than the experimental animals in all layers of CA۳ (p<۰.۰۵). It was also indicated that the neurons of CA۳ in experimental rats had more dendritic segments (۳۱.۲۵±۳.۳۳) than the controls (۲۷.۵±۲.۶۷), p<۰.۰۵. The dendritic branching density was higher in experimental rats relative to that found in the control rats. Conclusion Results of this study provide a neuroanatomical basis that may be relevant to the early reported enhancement of learning and memory abilities in offspring.  

Keywords:

Boswellia serrata , Hippocampus , Morphometry , pregnancy