The effect of vitamin C on mice hemolytic anemia induced by phenylhydrazine: an animal model study using histological changes in testis, pre-implantation embryo development and biochemical changes

Objective(s): The aim of the present study was to assess the effects of vitamin C (Vit C) on hemolytic anemia induced by phenylhydrazine (PHZ). Materials and Methods: Twenty-four healthy male mice were divided into four groups, randomly: Control group (۰.۱ ml/day, normal slaine, IP), PHZ group that received only PHZ ۸ mg/۱۰۰ g/۴۸ hr, IP, PHZ+Vit C group that received PHZ ۸ mg/۱۰۰ g/۴۸ hr, IP and Vit C ۱۰۰ mg/kg BW-۱/day by gavage and Vit C group that received ۱۰۰ mg/kg BW-۱/day Vit C by gavage. After ۳۵ days, germinal cells, RNA damage, sperm parameters, testis malondialdehyde (MDA) content, serum total antioxidant capacity (TAC), pre-implantation embryo development and mRNA levels of cyclin D۱ and c-myc in two-cell, and morula and blastocyst stages were assessed.  Results: Vit C reduced the RNA damage, enhanced sperm quality, promoted pre-implantation embryo development and improved testicular antioxidant and endocrine status (P<۰.۰۵). Vit C reduced cyclin D۱ expression and up-regulated c-myc mRNA level in two-cell, morula, and blastocyst embryonic cells. Conclusion: Vit C enhanced the fertilizing potential by ameliorating the endocrine status, antioxidant capacity, and sperm quality. Finally, the cyclin D۱ and c-myc gene expressions were regulated in PHZ+Vit C treated group that promoted the embryo development.

Keywords:

Hemolytic anemia , Histology , Mice , Testis , Vitamin C