Radiosensitizing effects of Sestrin۲ in PC۳ prostate cancer cells

Objective(s): The stress-responsive genes of Sestrin family are recognized as new tumor suppressor genes in breast carcinoma, however, the function of Sestrin family in human prostate cancer is not clear. Ionizing radiation (IR) is known to induce Sestrin gene expression in breast cancer cells. However, the response of Sestrin to IR has not been reported in PC۳ prostate cancer cells. Materials and Methods: Sestrin۲ expression in prostate cancer cell lines (PC۳, LNCaP clone FGC, and DU۱۴۵) was detected by Western blot and real-time PCR. Cell counting kit (CCK-۸) was used to detect cellular proliferation. The radiosensitivity of PC۳ cells was detected by clonogenic assay. Results: Sestrin۲ expression in prostate cancer cell lines (PC۳, LNCaP clone FGC, and DU۱۴۵) is low. In vitro assays indicated that over-expressing Sestrin۲ in human prostate cancer PC۳ inhibited tumor proliferation. In addition, elevated Sestrin۲ expression sensitized PC۳ cells to IR. Conclusion: We determined Sestrin۲ may function as a tumor suppressor through repressing proliferation, mediating sensitization to IR in PC۳ cells.

Keywords:

Cancer , Prostate , Radiation , Sensitization , Sestrin۲