The role of autophagy in advanced glycation end product-induced proliferation and migration in rat vascular smooth muscle cells

Objective(s): To investigate the role of autophagy in advanced glycation end products (AGEs)-induced proliferation and migration in rat vascular smooth muscle cells (VSMCs).Materials and Methods: After culture, VSMCs were treated with ۰, ۱, ۱۰, and ۱۰۰ μg/ml concentrations of AGEs. Autophagy specific protein light chain ۳ (LC۳)-I/II was determined by western blotting, autophagosomes were observed with electron microscopy, cell proliferation was quantified using the methyl thiazolyl tetrazolium (MTT) assay, and cell migration was evaluated using Transwell migration and scratch assays. Results: Compared to the control group, the level of LC۳- II/I in AGEs treatment group was up-regulated, and the number of autophagosomes was also increased. Furthermore, in concentration of ۱۰۰ μg/ml AGEs, the extent of proliferation and migration was significantly increased compared to the control group. However, pretreating cells with autophagy inhibitor ۳-MA could attenuate these effects.Conclusion: Our study demonstrated that AGEs-induced autophagy accelerated AGEs-stimulated proliferation and migration in VSMCs.

Keywords:

Advanced Glycation End Products , Autophagy , Cell Proliferation , Muscle , Smooth , Transcellular cell migration , Vascular