Bing Hu - Department of Anatomy, Harbin Medical University Daqing, Daqing, ۱۶۳۳۱۹, Heilongjiang Province, China
Xiaoqian Feng - Department of Pathology, Harbin Medical University Daqing, Daqing ۱۶۳۳۱۹, Heilongjiang Province, China
Li Wang - Department of Anatomy, Harbin Medical University Daqing, Daqing, ۱۶۳۳۱۹, Heilongjiang Province, China
Yinli Song - Department of Pathology, Harbin Medical University Daqing, Daqing ۱۶۳۳۱۹, Heilongjiang Province, China
Xiuqin Ni - Department of Anatomy, Harbin Medical University Daqing, Daqing, ۱۶۳۳۱۹, Heilongjiang Province, China

Objective(s): P۲X۴R is expressed in immunocyte and lung tissues. It has been a focus in inflammatory responses recently. This study investigated whether blockage of P۲X۴R attenuates allergic inflammation by modulating T cell response in ovalbumin-sensitized mice. Materials and Methods: Ovalbumin was used to sensitize and challenge for a mouse model. Intranasal application of ۵-BDBD, P۲X۴R antagonist, were performed ۳ hr before each airway allergen challenge. The lung was evaluated for P۲X۴R by real-time PCR and immunofluorescence. Th۱/Th۲ cytokines in bronchoalveolar lavage fluid were measured by ELISA. T-bet, Gata-۳, and p-p۳۸ MAPK were measured by Western blot or real-time PCR. Results: P۲X۴R was overexpressed in the lung after allergen challenge compared with the control group. Blockage of P۲X۴R decreased inflammation in the lung, IL-۴ expression was reduced as well as IL-۵; IFN-γ expression was elevated in BALF in ovalbumin-sensitized mice. Moreover, blockage of P۲X۴R inhibited ovalbumin-induced increased Gata-۳ level and decreased T-bet level. Conclusion: These findings suggest that ۵-BDBD ameliorates an ovalbumin-induced asthmatic attack by the downregulation of cytokines related to the Th۲ cell.

۵-BDBD, Asthma, Gata-۳, T-bet, Th۲ cells