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Title

MiR-۱۰۳ alleviates autophagy and apoptosis by regulating SOX۲ in LPS-injured PC۱۲ cells and SCI rats

Year: 1397
COI: JR_IJBMS-21-3_009
Language: EnglishView: 46
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Authors

Guowei Li - Department of Spine Surgery, The Fifth Affiliated Hospital of Sun Yat-Sen University, Zhuhai, Guangdong ۵۱۹۰۰۰, China
Tao Chen - Department of Spine Surgery, The Fifth Affiliated Hospital of Sun Yat-Sen University, Zhuhai, Guangdong ۵۱۹۰۰۰, China
Yingxian Zhu - Department of Anaesthesiology, The Fifth Affiliated Hospital of Sun Yat-Sen University, Zhuhai, Guangdong ۵۱۹۰۰۰, China
Xiaoyu Xiao - Department of Anaesthesiology, The Fifth Affiliated Hospital of Sun Yat-Sen University, Zhuhai, Guangdong ۵۱۹۰۰۰, China
Juyuan Bu - Department of Gastroenterological Surgery, The Fifth Affiliated Hospital of Sun Yat-Sen University, Zhuhai, Guangdong ۵۱۹۰۰۰, China
Zongwen Huang - Department of Spine Surgery, The Fifth Affiliated Hospital of Sun Yat-Sen University, Zhuhai, Guangdong ۵۱۹۰۰۰, China

Abstract:

Objective(s): Recent studies revealed that microRNAs (miRNAs) may play crucial roles in the responses and pathologic processes of spinal cord injury (SCI). This study aimed to investigate the effect and the molecular basis of miR-۱۰۳ on LPS-induced injuries in PC۱۲ cells in vitro and SCI rats in vivo. Materials and Methods: PC۱۲ cells were exposed to LPS to induce cell injuries to mimic the in vitro model of SCI. The expression of miR-۱۰۳ and SOX۲ in PC۱۲ cells were altered by transient transfections. Cell viability and apoptotic cell rate were measured by CCK-۸ assay and flow cytometry assay. Furthermore, Western blot analysis was performed to detect the expression levels of apoptosis- and autophagy- related proteins, MAPK/ERK pathway- and JAK/STAT pathway-related proteins. In addition, we also assessed the effect of miR-۱۰۳ agomir on SCI rats. Results: LPS exposure induced cell injuries in PC۱۲ cells. miR-۱۰۳ overexpression significantly increased cell viability, reduced cell apoptosis and autophagy, and opposite results were observed in miR-۱۰۳ inhibition. miR-۱۰۳ attenuated LPS-induced injuries by indirect upregulation of SOX۲. SOX۲ overexpression protected PC۱۲ cells against LPS-induced injuries, while SOX۲ inhibition expedited LPS-induced cell injuries. Furthermore, miR-۱۰۳ overexpression inhibited MAPK/ERK pathway and JAK/STAT pathway through upregulation of SOX۲. We also found that miR-۱۰۳ agomir inhibited cell apoptosis and autophagy in SCI rats. Conclusion: This study demonstrates that miR-۱۰۳ may represent a protective effect against cell apoptosis and autophagy in LPS-injured PC۱۲ cells and SCI rats by upregulation of SOX۲ expression.

Keywords:

Apoptosis , Autophagy , MAPK/ERK and JAK/STAT pathway , miR-۱۰۳ , SOX۲ , Spinal cord injury

Paper COI Code

This Paper COI Code is JR_IJBMS-21-3_009. Also You can use the following address to link to this article. This link is permanent and is used as an article registration confirmation in the Civilica reference:

https://civilica.com/doc/1295339/

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If you want to refer to this Paper in your research work, you can simply use the following phrase in the resources section:
Li, Guowei and Chen, Tao and Zhu, Yingxian and Xiao, Xiaoyu and Bu, Juyuan and Huang, Zongwen,1397,MiR-۱۰۳ alleviates autophagy and apoptosis by regulating SOX۲ in LPS-injured PC۱۲ cells and SCI rats,https://civilica.com/doc/1295339

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