Comparison of the efficacy of Piascledine and transforming growth factor β۱ on chondrogenic differentiation of human adipose-derived stem cells in fibrin and fibrin-alginate scaffolds

Publish Year: 1397
نوع سند: مقاله ژورنالی
زبان: English
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شناسه ملی سند علمی:

JR_IJBMS-21-2_014

تاریخ نمایه سازی: 27 مهر 1400

Abstract:

Objective(s):The aim of this study was to compare the chondrogenic induction potential of Piascledine and TGF-β۱ on adipose-derived stem cells (ADSCs) in fibrin and fibrin-alginate scaffolds.  Materials and Methods: Human subcutaneous adipose tissues were harvested from three patients who were scheduled to undergo liposuction. Isolated ADSCs were proliferated in a culture medium. Then, the cells were seeded in fibrin or fibrin-alginate scaffolds and cultured for ۱۴ days in a chondrogenic medium containing Piascledine, TGF-β۱, or both. The rate of cell proliferation and survival was evaluated by using MTT [۳-(۴, ۵-dimethylthiazolyl-۲)-۲, ۵-diphenyltetrazolium bromide] assay and the rate of the expression of type II collagen, aggrecan, and type X collagen genes was evaluated by real-time polymerase chain reaction (real-time PCR) method. Results: The MTT results showed that Piascledine is able to enhance the proliferation and survival of ADSCs in fibrin scaffolds in comparison to other groups (P<۰.۰۵). Real-time PCR evaluation revealed that the expression of type II collagen was higher in TGF- β۱groups, but the expression of aggrecan was higher in TGF-β۱ alone or along with Piascledine in fibrin-alginate scaffolds. Furthermore, the expression of type X collagen was lower in Piascledine alone or along with TGF-β۱ in fibrin scaffold. Conclusion: Piascledine can enhance the proliferation and differentiation of ADSCs in fibrin scaffolds.

Authors

Batul Hashemibeni

Department of Anatomical Sciences, Medical School, Isfahan University of Medical Sciences, Isfahan, Iran

Ali Valiani

Department of Anatomical Sciences, Medical School, Isfahan University of Medical Sciences, Isfahan, Iran

Mojtaba Esmaeili

Department of Anatomical Sciences, Medical School, Isfahan University of Medical Sciences, Isfahan, Iran

Mohamad Kazemi

Department of Molecular Biology, Medical School, Isfahan University of Medical Sciences, Isfahan, Iran

Maryam Aliakbari

Department of Anatomical Sciences, Medical School, Isfahan University of Medical Sciences, Isfahan, Iran

Farhad Golshan Iranpour

Department of Anatomical Sciences, Medical School, Isfahan University of Medical Sciences, Isfahan, Iran

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