Amany Ahmed Mohamed Abd-Allah - Department of Clinical Pathology, Faculty of Veterinary Medicine, Zagazig University, Zagazig, Egypt
Nasr Abdelwahab Mohamed Nasr El-Deen - Department of Clinical Pathology, Faculty of Veterinary Medicine, Zagazig University, Zagazig, Egypt
wafaa mohamed - lecturer of clinical pathology,faculty of veterinary medicine,zagazig university
Fatma Mohamed Naguib - MVSc of Clinical Pathology, Faculty of Veterinary Medicine, Zagazig University, Zagazig, Egypt

Objective(s): Reactive oxygen species (ROS)-produced oxidative disorders were involved at the pathophysiology of many inflammatory processes via the generation of pro-inflammatory cytokines and antioxidant defense system suppression. Although herbal antioxidants as mono-therapy relief many inflammatory diseases including, autoimmunity rheumatoid arthritis, but as combination therapy with other proven anti-inflammatory drugs in order to decreasing their toxic impacts has not yet been studied clearly, especially against chemical substances that’s induced local inflammation with characteristic edema. Materials and Methods: Grape seeds extract (GSE) at a concentration of ۴۰ mg/kg B. wt alone or in combination with indomethacin (Indo.) at a dose of ۵ mg/Kg B. wt orally given for ۱۰ days prior (gps VI, VII, VIII) or as a single dose after edema induction (gps IX, X, XI) in rat's left hind paw by sub-planter single injection of ۰.۱ carrageenan: saline solution (۱%) (gp. V) to assess the prophylactic and therapeutic anti-inflammatory activities of both through  the estimation of selective inflammatory mediators and oxidative damage-related biomarkers as well as tissue mast cell scoring. Furthermore, both substances were given alone (gps II, III, IV) for their  blood, liver and kidney safety evaluation comparing with negative control rats (gp. I) which kept without medication. Results: A marked reduction on the inflammatory mediators, edema volume and oxidative byproducts in edema bearing rats' prophylactic and treated with grape seeds extract and indomethacin was observed. Indomethacin found to induce some toxicological impacts which minimized when administered together with GSE. Conclusion: GSE is a safe antioxidant agent with anti-inflammatory property.

Gastritis, TNF-α, IgE, Indomethacin, Proanthocyanidins, Toluidine-blue