Association of the gene expression variation of tumor necrosis factor-α and expressions changes of dopamine receptor genes in progression of diabetic severe foot ulcers

Objective(s):Regulation of pro-inflammatory factors such as TNF-𝛼, which are secreted by the immune cells through induction of their several receptors including dopamine receptors (especially DRD۲ and DRD۳) is one of the noticeable problems in diabetic severe foot ulcer healing. This study was conducted to evaluate the alteration of TNF-𝛼 in plasma as well as DRD۲ and DRD۳ changes in PBMCs of diabetics with severe foot ulcers. Materials and Methods: Peripheral blood samples were collected from ۳۱ subjects with ulcers, ۲۹ without ulcers, and ۲۵ healthy individuals. Total mRNA was extracted from PBMCs for the study of DRD۲, DRD۳, and TNF-𝛼 gene expression variations. Expression patterns of these genes were evaluated by real-time PCR. Consequently, concentration of TNF-𝛼 was investigated in plasma. Results: Significant decrease in gene expression and plasma concentration of TNF-𝛼 in PBMCs was observed in both patient groups at P Conclusion: We concluded that DRD۲ and DRD۳ expression alteration and presence of new DRD۳ transcripts can be effective in reduction of TNF-α expression as a pro-inflammatory factor. Performing complementary studies, may explain that variations in DRD۲ and DRD۳ are prognostic and effective markers attributed to the development of diabetes severe foot ulcers.

Keywords:

Diabetic severe foot ulcers , Dopamine receptor , Medical genetics , Regenerative medicine , Tumor necrosis factor-α