Sri Agus Sudjarwo - Department of Pharmacology, Faculty of Veterinary Medicine, Airlangga University, Surabaya ۶۰۱۱۵ Indonesia
Koerniasari Eraiko - Department of Conservative Dentistry, Faculty of Dentistry, Airlangga University, Surabaya ۶۰۱۱۵ Indonesia
Giftania Wardani Sudjarwo - Department of Pharmacy Biology, Faculty of Pharmacy, Hang Tuah University, Surabaya ۶۰۱۱۵ Indonesia
Koerniasari . - Study Program of Environmental Health, Polytechnic of Health, Surabaya ۶۰۱۱۵ Indonesia

Objective(s): In this study, we investigated the protective effects of piperine on lead acetate-induced renal damage in rat kidney tissue. Materials and Methods: Forty male rats were divided into ۵ groups: negative control (rats were given aquadest daily), positive control (rats were given lead acetate ۳۰ mg/kg BW orally once a day for ۶۰ days), and the treatment group (rats were given piperine ۵۰ mg; ۱۰۰ mg and ۲۰۰ mg/kg BW orally once a day for ۶۵ days, and on ۵th day, were given lead acetate ۳۰ mg/kg BW one hr after piperine administration for ۶۰ days). On day ۶۵ levels of blood urea nitrogen (BUN), creatinine, malondialdehyde (MDA), Superoxide Dismutase (SOD), and Glutathione Peroxidase (GPx) were measured. Also, kidney samples were collected for histopathological studies. Results: The results revealed that lead acetate toxicity induced a significant increase in the levels of BUN, creatinine, and MDA; moreover, a significant decrease in SOD and GPx. Lead acetate also altered kidney histopathology (kidney damage, necrosis of tubules) compared to the negative control. However, administration of piperine significantly improved the kidney histopathology, decreased the levels of BUN, creatinine, and MDA, and also significantly increased the SOD and GPx in the kidney of lead acetate-treated rats. Conclusion: From the results of this study it was concluded that piperine could be a potent natural herbal product exhibiting nephroprotective effect against lead acetate induced nephrotoxicity in rats.

Antioxidants, Lead acetate, Nephrotoxicity, Piperine, Protective