The effect of platelet-rich plasma on human mesenchymal stem cell-induced bone regeneration of canine alveolar defects with calcium phosphate-based scaffolds

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1396

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مقاله ژورنالی

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English

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شناسه ملی سند علمی:

JR_IJBMS-20-10_009

تاریخ نمایه سازی: 28 مهر 1400

Abstract:

Objective(s): Autologous bone transplantation known as the “gold standard” to reconstruction of osseous defects has known disadvantages. This study was designed to explore the effects of hydroxy-apatite/tricalcium-phosphate (HA/TCP) and platelet-rich plasma (PRP) on the osteogenesis ability of human adipose-derived mesenchymal stem cells (hAdMSCs) in vitro and in vivo. Materials and Methods: hAdMSCs were incubated with HA/TCP granules and/or PRP in vitro and then, cell proliferation and differentiation was assessed by MTT assay, AZR S staining and SEM examination. In vivo, four cylindrical defects were drilled in the mandibular bones of ۵ mongrel dogs and divided randomly into the following groups: I-autologous crushed bone, II- no filling material, III- HA/TCP and PRP, IV- PRP-enriched hAdMSCs seeded on HA/TCP granules. Inserted hAdMSCs were labeled to trace their contribution to bone tissue regeneration. Finally, cell tracing and tissue regeneration were evaluated by immunohistochemistry and histomorphometry methods, respectively.    Results: In vitro, co-incubation with HA/TCP granules significantly reduced proliferation and osteogenic differentiation ability of hAdMSCs; while PRP application promoted these capacities (P<۰.۰۵). In vivo, PRP-enriched hAdMSCs seeded on HA/TCP granules induced considerable bone formation in osseous defects (P<۰.۰۵). It was obviously shown that hAdMSCs were incorporated into the newly-formed bone.    Conclusion: Based on this study, application of stem cells could offer a helpful therapeutic tool in bone tissue regeneration. Although inserted hAdMSCs were identifiable throughout the newly-formed bone tissue, their few number could be an indicator of indirect role of hAdMSCs in tissue regeneration.

Authors

ReihanehShafieian
Reihaneh Shafieian

Department of Anatomy and Cell Biology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran

MaryamMoghaddam Matin
Maryam Moghaddam Matin

Department of Biology, Faculty of Sciences, Ferdowsi University of Mashhad, Mashhad, Iran

AminRahpeyma
Amin Rahpeyma

Oral and Maxillofacial Diseases Research Center, School of Dentistry, Mashhad University of Medical Sciences, Mashhad, Iran

AlirezaFazel
Alireza Fazel

Department of Anatomy and Cell Biology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran

HamidehSalari Sedigh
Hamideh Salari Sedigh

Department of Clinical Sciences, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran

ArianeSadr-Nabavi
Ariane Sadr-Nabavi

Department of Genetics, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran

HalimehHassanzadeh
Halimeh Hassanzadeh

Department of Biology, Faculty of Sciences, Ferdowsi University of Mashhad, Mashhad, Iran

AlirezaEbrahimzadeh-Bideskan
Alireza Ebrahimzadeh-Bideskan

Department of Anatomy and Cell Biology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran

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