Staging of colorectal cancer using serum metabolomics with ۱HNMR Spectroscopy

Objective(s): Determination of stages of colon cancer is done by biopsy usually after surgery. Metabolomics is the study of all the metabolites using LC-MS and ۱HNMR spectroscopy with chemometric techniques. The stages of colon cancer were detected from patients' sera using ۱HNMR. Materials and Methods: Five ml blood was collected from ۱۶ confirmed patients referred for colonoscopy.  One group of eight patients were diagnosed with stage ۰ to I colon cancer and the second group of ۸ patients with II-IV stage colon cancer.  Sera were sent for ۱HNMR. The differentiating metabolites were identified using HMDB  and the metabolic cycles from Metaboanalyst. Results: Six metabolites of which pyridoxine levels lowered, and glycine, cholesterol, taurocholic acid, cholesteryl ester and deoxyinosine increased. Conclusion: The different stages of cancer were identified by the main metabolic cycles such as primary bile acid biosynthesis, purine and vitamin B metabolic pathways and the glutathione cycle.

Keywords:

Colon cancer , HNuclear Magnetic Resonance Spectroscopy , Metabolomics , Serum , Staging