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CFP۱۰: mFcγ۲ as a novel tuberculosis vaccine candidate increases immune response in mouse

عنوان مقاله: CFP۱۰: mFcγ۲ as a novel tuberculosis vaccine candidate increases immune response in mouse
شناسه ملی مقاله: JR_IJBMS-20-2_003
منتشر شده در در سال 1396
مشخصات نویسندگان مقاله:

Ali Asghar Baghani - Antimicrobial Resistance Research Center, Bu-Ali Research Institute, Mashhad University of Medical Sciences, Mashhad, Iran
Saman Soleimanpour - Antimicrobial Resistance Research Center, Bu-Ali Research Institute, Mashhad University of Medical Sciences, Mashhad, Iran
Hadi Farsiani - Antimicrobial Resistance Research Center, Bu-Ali Research Institute, Mashhad University of Medical Sciences, Mashhad, Iran
Arman Mosavat - HIV/AIDS, HTLV and Viral Hepatitis Research Center, Iranian Academic Center for Education, Culture and Research (ACECR), Mashhad, Iran
Masoud Yousefi - Antimicrobial Resistance Research Center, Bu-Ali Research Institute, Mashhad University of Medical Sciences, Mashhad, Iran
Zahra Meshkat - Antimicrobial Resistance Research Center, Bu-Ali Research Institute, Mashhad University of Medical Sciences, Mashhad, Iran
Seyed Abdolrahim Rezaee - Immunology Research Center, Inflammation and Inflammatory Diseases Division, Medical school, Mashhad University of Medical Sciences, Mashhad, Iran
Saeid Amel Jamehdar - Antimicrobial Resistance Research Center, Bu-Ali Research Institute, Mashhad University of Medical Sciences, Mashhad, Iran
Mohammad Reza Akbari Eydgahi - Biotechnology Research Center, Semnan University of Medical Sciences, Semnan, Iran
Hamid Sadeghian - Organic Chemistry, Department of Laboratory Sciences, Mashhad University of Medical Sciences, Mashhad, Iran
Kiarash Ghazvini - Antimicrobial Resistance Research Center, Bu-Ali Research Institute, Mashhad University of Medical Sciences, Mashhad, Iran

خلاصه مقاله:
Objective(s): Despite treatment with antibiotics and vaccination with BCG, tuberculosis (TB) is still considered as one of the most important public health problems in the world. Therefore, designing and producing a more effective vaccine against TB seems urgently. In this study, immunogenicity of a fusion protein which consisting or comprising CFP-۱۰ from Mycobacterium tuberculosis and the Fc-domain of  mouse IgG۲a was evaluated as a novel subunit vaccine candidate against TB. Materials and Methods: The genetic constructs were cloned in pPICZαA expression vector and recombinant vectors (pPICZαA-CFP-۱۰: Fcγ۲a and pPICZαA-CFP-۱۰:His) were transformed into Pichia pastoris. To evaluate the expression of recombinant proteins, SDS-PAGE and immunoblotting were used. The immunogenicity of recombinant proteins, with and without BCG were assessed in BALB/c mice and specific cytokines against recombinant proteins (IFN-γ, IL-۱۲, IL-۴, IL-۱۷ and TGF-β) were evaluated. Results: The levels of IFN-γ and IL-۱۲ in mice that received recombinant proteins was higher than the control groups (BCG and PBS). Thus, both recombinant proteins (CFP-۱۰:Fcγ۲a and CFP-۱۰:His) could excite good response in Th۱-cells. The Fc-tagged protein had a stronger Th۱ response with low levels of IL-۴, as compared to CFP-۱۰:His. However, the highest level of Th۱ response was observed in groups that were vaccinated with BCG (prime) and then received recombinant protein CFP-۱۰: Fcγ۲a (booster). Conclusion: The results demonstrated that binding mice Fc-domain to CFP-۱۰ protein can increase the immunogenicity of the subunit vaccine. Further studies, might be able to design and produce a new generation of subunit vaccines based on the Fc-fused immunogen.

کلمات کلیدی:
CFP-۱۰, Fcγ۲a, Immunogenicity, Mycobacterium tuberculosis, Subunit vaccine

صفحه اختصاصی مقاله و دریافت فایل کامل: https://civilica.com/doc/1295768/