Cisplatin cytotoxicity is dependent on mitochondrial respiration in Saccharomyces cerevisiae
Publish place: Iranian Journal of Basic Medical Sciences، Vol: 20، Issue: 1
Publish Year: 1396
نوع سند: مقاله ژورنالی
زبان: English
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JR_IJBMS-20-1_013
تاریخ نمایه سازی: 28 مهر 1400
Abstract:
Objective(s): To understand the role of mitochondrial respiration in cisplatin sensitivity, we have employed wild-type and mitochondrial DNA depleted Rho۰ yeast cells. Materials and Methods: Wild type and Rho۰ yeast cultured in fermentable and non-fermentable sugar containing media, were studied for their sensitivity against cisplatin by monitoring growth curves, oxygen consumption, pH changes in cytosol/mitochondrial compartments, reactive oxygen species production and respiratory control ratio. Results: Wild-type yeast grown on glycerol exhibited heightened sensitivity to cisplatin than yeast grown on glucose. Cisplatin (۱۰۰ μM), although significantly reduced the growth of wild- type cells, only slightly altered the growth rate of Rho۰ cells. Cisplatin treatment decreased both pHcyt and pHmit to a similar extent without affecting the pH difference. Cisplatin dose-dependently increased the oxidative stress in wild-type, but not in respiration-deficient Rho۰ strain. Cisplatin decreased the respiratory control ratio. Conclusion: These results suggest that cisplatin toxicity is influenced by the respiratory capacity of the cells and the intracellular oxidative burden. Although cisplatin per se slightly decreased the respiration of yeast cells grown in glucose, it did not disturb the mitochondrial chemiosmotic gradient.
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Authors
Santhipriya Inapurapu
Institute of Genetics & Hospital for Genetic Diseases, Osmania University, Hyderabad, Telangana, India-۵۰۰۰۱۶
Karunakar Rao Kudle
Department of Biochemistry, Osmania University, Hyderabad, Telangana, India- ۵۰۰۰۰۷
Sreedhar Bodiga
Department of Biochemistry, Kakatiya University, Warangal, Telangana, India -۵۰۶۰۰۹
Vijaya Lakshmi Bodiga
Institute of Genetics & Hospital for Genetic Diseases, Osmania University, Hyderabad, Telangana, India-۵۰۰۰۱۶
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