In vitro assesment of anti-inflammatory activities of coumarin and Indonesian cassia extract in RAW۲۶۴.۷ murine macrophage cell line

Objective(s): Inflammation is an immune response toward injuries. Although inflammation is healing response, but in some condition it will lead to chronic disease such as rheumatoid arthritis, inflammatory bowel disease, atherosclerosis, Alzheimer’s and various cancer. Indonesian cassia (Cinnamomum burmanni C. Nees & T. Ness) known to contain coumarin, is widely used for alternative medicine especially as an antiinflammator.This study was conducted to determine the anti-inflammatory properties of coumarin and Indonesian cassia extract (ICE) in LPS-induced RAW۲۶۴.۷ cell line. Materials and Methods: The cytotoxic assay of coumarin and ICE against RAW۲۶۴.۷ cells was conducted using MTS (۳-(۴,۵-dimethylthiazol-۲-yl)-۵-(۳-carboxymethoxyphenyl)-۲-(۴-sulfophenyl)-۲H-tetrazolium). The anti-inflammatory potential was determined using LPS-induced RAW ۲۶۷.۴ macrophages cells to measure inhibitory activity of both compounds on production of nitric oxide (NO), prostaglandin E۲ (PGE۲), and also cytokines such as interleukin-۶ (IL-۶), interleukin-۱β  (IL-۱β)  and TNF-α. Results: Coumarin ۱۰ µM and ICE ۱۰ µg/ml were nontoxic to the RAW۲۶۴.۷ cells. Both of coumarin and ICE were capable to reduce the PGE۲, TNF-α, NO, IL-۶, and IL-β level in LPS-induced RAW۲۶۴.۷ cells. Coumarin had higher activity to decrease PGE۲ and TNF-α, whilst ICE had higher activity to inhibit NO, IL-۶, and IL-β levels. Conclusion: Coumarin and ICE possess anti-inflammatory properties through inhibition of PGE۲ and NO along with pro-inflammatory cytokines TNF-α, IL-۶, IL-۱β production.

Keywords:

Coumarin , Cytokines , Indonesian cassia extract , Inflammation , RAW۲۶۴.۷