Movento influences development of granulosa cells and ovarian follicles and FoxO۱ and Vnn۱ gene expression in BALB/c mice

Objective(s): Pesticides has wide range of infertility in female reproductive. This study was done to evaluate the effect of movento pesticide on development of granulosa cells and ovarian follicles and FoxO۱ and Vnn۱ gene expression in BALB/c mice. Materials and Methods: In this study ۴۰ healthy BALB/c mice ۵-۶ weeks age were used. Animals were randomly allocated into four groups. Control (without any intervention), three experimental groups received ۲۵, ۵۰ and ۱۰۰ mg/kg movento dissolved in PBS by gavage for ۲۱ days. Animals scarified after three weeks. For determining the effects of movento on granulosa cells in culture, treatments were conducted to movento (۱۲۵, ۲۵۰, ۵۰۰ μg/ml) for ۲۴ hr. We surveyed the expression of the FoxO۱ and Vnn۱ in granulosa cells in vitro, and its relation to cell death by flowcytometer and DAPI. Levels of FoxO۱ and Vnn۱ were analyzed by real-time PCR. Results: Exposure to movento significantly decreased ovarian weight and the number of primary, secondary and antral follicles. Further, treatment with different concentration of movento induced apoptosis on granulosa cells. Gene expression analysis showed the transcriptional expression of FoxO۱ and vnn۱ in granulosa cells. Level of Vnn۱ mRNA in granulosa cells was decreased in granulosa cells and expression of FoxO۱ significantly increased in treated groups in compare to controls (P-value <۰.۰۵). Conclusion: Exposure to movento significantly reduced the number of follicles and increased apoptosis of granulosa cells leading disruption of the reproductive system. Also movento reduced expression of Vnn۱ and increased FoxO۱ genes in a dose dependent manner.

Keywords:

Apoptosis , FoxO۱ , Granulosa cell , Movento , Ovary , Vnn۱