Antifungal activity of Zataria multiflora essential oil-loaded solid lipid nanoparticles in-vitro condition
Publish Year: 1395
نوع سند: مقاله ژورنالی
زبان: English
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شناسه ملی سند علمی:
JR_IJBMS-19-11_012
تاریخ نمایه سازی: 30 مهر 1400
Abstract:
Objective(s): The aim of the present study was to prepare, characterize, and evaluate solid lipid nanoparticles (SLNs) containing Zataria multiflora essential oil (ZEO). Materials and Methods: In this study, Z. multiflora essential oil-loaded solid lipid nanoparticles (ZE-SLNs) were prepared to improve its efficiency in controlling some fungal pathogens. SLNs containing Z. multiflora essential oil were prepared by high shear homogenization and ultra sound technique. ZEO-SLNs contained ۰.۰۳% ZEO in ۵% of lipid phase (Glyceryl monostearate-GMS and Precirol® ATO ۵).Tween ۸۰ and Poloxamer ۱۸۸ (۲.۵% w/v) were used as surfactant in the aqueous phase. The antifungal efficacy of ZE-SLNs and ZEO was compared under in vitro conditions. Results:The particle size of ZE-SLNs was around ۲۵۵.۵±۳ nm with PDI of ۰.۳۶۹±۰.۰۵ and zeta potential was about -۳۷.۸±۰.۸ mV. Encapsulation efficacy of ZE-SLNs in crystalline form was ۸۴±۰.۹۲%. The results showed that the ZEO and ZE-SLNs had ۵۴ and ۷۹% inhibition on the growth of fungal pathogens, respectively. The minimum inhibitory concentration (MIC) under in vitro conditions for the ZEO on the fungal pathogens of Aspergillus ochraceus, Aspergillus niger, Aspergillus flavus, Alternaria solani, Rhizoctonia solani, and Rhizopus stolonifer was ۳۰۰, ۲۰۰, ۳۰۰, ۲۰۰, ۲۰۰ and ۲۰۰ ppm, respectively, for ZE-SLNs, it was ۲۰۰, ۲۰۰, ۲۰۰, ۱۰۰, ۵۰ and ۵۰ ppm. The antifungal efficacy of ZE-SLNs was significantly more than ZEO. Conclusion: Our results showed that the SLNs were suitable carriers for Z. multiflora essential oil in controlling the fungal pathogens and merits further investigation.
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Authors
Mahboobeh Nasseri
Department of Horticulture Science, Faculty of Agriculture, Ferdowsi University of Mashhad, Iran
Shiva Golmohammadzadeh
Nanotechnology Research Center, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
Hossein Arouiee
Department of Horticulture Science, Faculty of Agriculture, Ferdowsi University of Mashhad, Iran
Mahmoud Reza Jaafari
Biotechnology Research Center, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
Hossein Neamati
Department of Horticulture Science, Faculty of Agriculture, Ferdowsi University of Mashhad, Iran
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